9002-08-8 Usage
Uses
Trypsinogen from bovine pancreas is suitable for use in:the secondary structure analysis of proteins in H2O solution using single-pass attenuated total reflection Fourier transform infrared (ATR-FT-IR) microscopytuning and calibration of electrospray ionization quadrupole time-of-flight (ESI-Q-TOF) mass spectrometerthe secondary structure analysis of proteins by infrared (IR) spectroscopySDS-PAGE as a molecular weight standard (24kDa)
General Description
Trypsinogen is a proenzyme (zymogen) that is activated to form trypsin. It is synthesized in the pancreas and activated by enterokinase once it reaches the lumen of the small intestine. Bovine trypsinogen is a single polypeptide chain of 229 amino acids that is cross linked by six disulfide bridges. Enterokinase cleaves a hexapeptide to from the NH2 terminus of trypsinogen at the Lys6 - Ile7 peptide bond and activates it. Trypsin, thus formed, autocatalytically activates more trypsinogen to trypsin. This native form of trypsin is called β-trypsin, which undergoes autolysis at Lys131 - Ser132 resulting in α-trypsin that is held together by disulfide bridges. Trypsin is a serine protease with His46 and Ser183 at the active site. The pH optimum of trypsin is 7 - 9.
Biochem/physiol Actions
Hereditary pancreatitis was shown to be caused by a Arg-His substitution at residue 117 of trypsinogen causing auto-activation of trypsinogen to trypsin.
Check Digit Verification of cas no
The CAS Registry Mumber 9002-08-8 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,0 and 2 respectively; the second part has 2 digits, 0 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 9002-08:
(6*9)+(5*0)+(4*0)+(3*2)+(2*0)+(1*8)=68
68 % 10 = 8
So 9002-08-8 is a valid CAS Registry Number.