9004-02-8

Basic information

• Product Name: LIPOPROTEIN LIPASE
• Synonyms: LIPOPROTEIN LIPASE;DIACYL-GLYCEROL ACYLHYDROLASE;DIACYLGLYCEROL LIPASE;EC 3.1.1.34;EC: 3.1.1.3.4;Lipase,lipoprotein;lipoproteinlipasef.chromobact.visc.;Lipoprotein Lipase Chromobacterium viscosum
• CAS NO: 9004-02-8
• Molecular Formula: NULL
• Molecular Weight: 0
• EINECS: 232-669-1
• Product Categories: Enzymes;HydrolasesMetabolomics;Lipid MetabolismApplication Index;3.1.x.x Acting on esters;3.x.x.x Hydrolases;Enzyme-Mediated Synthesis;General Metabolic Enzymes;Organic Synthesis and Immobilized Enzymes;Organic SynthesisEnzyme Class Index;Specialty Synthesis
• Mol File: 9004-02-8.mol
• Article Data: 0

Chemical Properties

• Appearance: yellow-brown/powder
• Storage Temp.: 2-8°C
• Merck: 13,5534
• CAS DataBase Reference: LIPOPROTEIN LIPASE(CAS DataBase Reference)
• NIST Chemistry Reference: LIPOPROTEIN LIPASE(9004-02-8)
• EPA Substance Registry System: LIPOPROTEIN LIPASE(9004-02-8)

Safety Data

• Safety Statements: 22-24/25-36/37
• WGK Germany: 3
• RTECS: TO9776500
• F: 3-10
• Hazardous Substances Data: 9004-02-8(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 9004-02-8 differently. You can refer to the following data:
1. Lipoprotein Lipase from Burkholderia sp. has been used in the subcellular fractionation of mussel Mytilus galloprovincialis to identify the biomolecule attached to cytosolic fraction of okodaic acid. It has also been used to test its effect on serum amyloid A induced granulocyte colony-stimulated factor (G-CSF) expression in response to bacterial lipoprotein.
2. Lipoprotein Lipase from bovine milk has been used:as a supplement to test its effect on DiI (1,1′-dioctadecyl-3,3,3′-tetramethyl-indocarbocyanine perchlorate)- very-low-density lipoprotein (VLDL) uptake in breast cancer MDA-MB-231 cells to treat human brain microvascular endothelial cells (HBMECs) for the lipolysis of triglyceride-rich lipoproteins (TGRL)to test its effect on gene expression in normal human astrocytes

General Description

Lipoprotein Lipase (LPL) is a glycerol ester hydrolase. Several bacteria that produce LPL, belongs to the genus Pseudomonas, Serratia and Mucor.

Biochem/physiol Actions

Malic dehydrogenase catalyzes the dehydrogenation of L-malate by NAD+.

Purification Methods

Purify the lipase by affinity chromatography on heparin-Sepharose; Ki 0.026mM for very low density lipoprotein. It is inhibited by 2-mercaptoethanol, Cys, Ca, Hg, Mg and Mn ions. Protamine sulfate, 1mg of bovine serum albumin/mL or in 50% glycerol at -70o, stabilises the lipase for several days. 60% loss of activity occurs at 0o/1hour in the presence of 1% of bovine serum albumin. [Shirai et al. Biochim Biophys Acta 665 504 1981.] Lipoproteins (from human plasma). Individual human plasma lipid peaks are removed from plasma by ultracentrifugation; then they are separated and purified by agarose-column chromatography. Fractions are characterised immunologically, chemically, electrophoretically and by electron microscopy. [Rudel et al. Biochem J 13 89 1974.]