9012-56-0

Basic information

• Product Name: EC 3.5.1.4
• Synonyms: EC 3.5.1.4;ACYLAMIDE AMIDOHYDROLASE;ACYLASE;ACRYLAMIDE AMIDOHYDROLASE;AMIDASE;AMIDASE BS;AMIDASE, PSEUDOMONAS AERUGINOSA;AMIDASE PSEUDOMONAS AERUGINOSA RECOM-&
• CAS NO: 9012-56-0
• Molecular Weight: 0
• EINECS: 232-736-5
• Mol File: 9012-56-0.mol
• Article Data: 0

Chemical Properties

• Appearance: yellow/solution (slightly hazy)
• Storage Temp.: −20°C
• CAS DataBase Reference: EC 3.5.1.4(CAS DataBase Reference)
• NIST Chemistry Reference: EC 3.5.1.4(9012-56-0)
• EPA Substance Registry System: EC 3.5.1.4(9012-56-0)

Safety Data

• Hazard Codes: Xn
• Statements: 42/43-52/53
• Safety Statements: 22-36/37-45-61
• WGK Germany: 3
• F: 10
• Hazardous Substances Data: 9012-56-0(Hazardous Substances Data)

Usage

Uses

The importance of these hydrolases in biotechnology is growing rapidly, because their potential applications span through chemical and pharmaceutical industries as well as in bioremediation. Immobilized amidase can be used efficiently for production of acrylic acid from acrylamide, thus converting a toxic ambient contaminant into widely used industrial raw material. Amidases are potential treatments for human immunodeficiency virus and malaria. They may be used to eliminate metal ions in wastewater .

Definition

Any deamidizing hydrolase that catalyses the hydrolysis of mono carboxylic amides, yielding the free acid and ammonia.

Biochem/physiol Actions

The amidase from Pseudomonas aeruginosa isa 6 × 38-kDa enzyme that catalyzes the hydrolysis of a small range of short aliphatic amides. Each amidase monomer is formed by a globular four-layer αββα sandwich domain with an additional 81-residue long C-terminal segment .