9012-69-5

Basic information

• Product Name: PHOSPHORYLASE B
• Synonyms: ALPHA-GLUCAN PHOSPHORYLASE;1,4-ALPHA-D-GLUCAN: ORTHOPHOSPHATE ALPHA-D-GLUCOSYLTRANSFERASE;GLYCOGEN PHOSPHORYLASE;EC 2.4.1.1;PHOSPHORYLASE B CROSS-LINKED RABBIT;PHOSPHORYLASE B FROM RABBIT MUSCLE*FOR S DS GEL;PHOSPHORYLASE B FROM RABBIT MUSCLE, LYOP H., ~10 U/MG;α-glucan phosphorylase
• CAS NO: 9012-69-5
• Molecular Weight: 0
• EINECS: 232-737-0
• Mol File: 9012-69-5.mol
• Article Data: 0

Chemical Properties

• Appearance: light yellow/powder
• Storage Temp.: 2-8°C
• Water Solubility: Soluble in water
• CAS DataBase Reference: PHOSPHORYLASE B(CAS DataBase Reference)
• NIST Chemistry Reference: PHOSPHORYLASE B(9012-69-5)
• EPA Substance Registry System: PHOSPHORYLASE B(9012-69-5)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10-21
• TSCA: Yes
• Hazardous Substances Data: 9012-69-5(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 9012-69-5 differently. You can refer to the following data:
1. Phosphorylase b is used to study the conversion mechanism of inactive phosphorylase b to active phosphorylase in muscle. Phosphorylase b is used to study which factors influence the conversion of phosphorylase b to phosphorylase a such as temperature, AMP, fluoride and detergents. It is used to study phosphorylase b deficiency mutations.
2. Phosphorylase b from rabbit muscle has been used as a molecular weight marker in 12% polyacrylamide gel for keratinase, and stress-70 protein.

General Description

Phosphorylase b is a dimer, usually exists in inactive form in the skeletal muscles. Equilibrium exists between an active relaxed (R) state and a less active tense (T). Phosphorylase b favors the T state. The enzyme possesses three domains, the N-terminal domain, glycogen-binding domain and the C-terminal domain.

Biochem/physiol Actions

Phosphorylase b is a non-active form and is present in resting muscle. Phosphorylase b kinase activity increases significantly when the Mg2+:ATP ratio exceeds 1. The breakdown of ATP during muscle contraction is thought to trigger in vivo conversion of phosphorylase b into a. Phosphorylase b is activated by inosine monophosphate.