9012-90-2

Basic information

• Product Name: DNA polymerase
• Synonyms: DNA POLYMERASE;DNA POLYMERASE 1, KLENOV FRAGMENT;DNA POLYMERASE 1, KLENOW FRAGMENT;DNA POLYMERASE 1, LARGE FRAGMENT;DNA POLYMERASE I;DNA POLYMERASE I, KLENOW;DNA POLYMERASE I (KLENOW FRAGMENT);DNA POLYMERASE I, LARGE FRAGMENT
• CAS NO: 9012-90-2
• Molecular Formula: NULL
• Molecular Weight: 0
• EINECS: 232-741-2
• Product Categories: 2.7.x.x Phosphorus containing groups;Nucleic Acid Labeling Enzymes and ReagentsApplication Index;PCRMolecular Biology;Routine AmplificationPlant Molecular Biology;Routine PCR AmplificationEnzyme Class Index;2.x.x.x Transferases;Molecular Biology;Molecular Biology Tested;Nucleic Acid Amplification;Nucleic Acid Detection and Hybridization;PCR/Amplification;Modifying EnzymesMolecular Biology;Nucleic Acid Labeling Enzymes and Reagents;Modifying EnzymesApplication Index;Molecular Biology Enzymes
• Mol File: 9012-90-2.mol
• Article Data: 0

Chemical Properties

• Appearance: colorless/buffered aqueous glycerol solution
• Storage Temp.: −20°C
• CAS DataBase Reference: DNA polymerase(CAS DataBase Reference)
• NIST Chemistry Reference: DNA polymerase(9012-90-2)
• EPA Substance Registry System: DNA polymerase(9012-90-2)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38-43
• Safety Statements: 26-36-24/25-36/37
• WGK Germany: 3
• F: 10
• TSCA: Yes
• Hazardous Substances Data: 9012-90-2(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 9012-90-2 differently. You can refer to the following data:
1. Taq DNA Polymerase from Thermus aquaticus has been used in the process of DNA extraction (during gene amplification and sequencing). It has been used in genotyping. It has also been used in polymerase chain reaction (PCR) to study the constitutive production of epithelial neutrophil activating peptide 78 (ENA-78) and interleukin-8 (IL-8).
2. REDTaq? Genomic DNA Polymerase has been used as a component of the polymerase chain reaction (PCR) mix for PCR amplification. It has also been used as a component of the preamplification mix for PCR in amplified fragment length polymorphism (AFLP) analysis of Fusarium oxysporum sp. isolates.
3. For routine PCR amplifications REDTaq? DNA Polymerase has been used in polymerase chain reaction (PCR) and reverse transcription-polymerase chain reaction (RT-PCR) analysis.

General Description

Klenow enzyme is the large, C-terminal fragment (Mr 76,000) of E.coli DNA polymerase I, which can be obtained by subtilisin treatment of intact DNA polymerase I. It retains the 5′→3′ polymerase and the 3′→5′ exonuclease activities of intact DNA polymerase I, but lacks the 5′→3′ exonuclease activity of the native enzyme. The enzyme catalyzes the addition of mononucleotides from deoxynucleoside-5′-triphosphates to the 3′-hydroxyl terminus of a primer/template DNA. This property is used to synthesize DNA complementary to single-stranded DNA templates.Klenow Enzyme is formed by subtilisin treatment of DNA polymerase I.

Biochem/physiol Actions

Taq polymerase catalyzes oligonucleotide primer-driven, DNA template dependent incorporation of dNTPs into complimentary DNA strands. It displays both 5′ to 3′ polymerase and exonuclease activities.