9014-08-8

Basic information

• Product Name: ENOLASE
• Synonyms: 2-Phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase;Enolase from baker's yeast (S. cerevisiae),2-Phospho-D-glycerate hydro-lyase, Phosphopyruvate hydratase;Enolase, 2-phospho-D-glycerate hydro-lyase;Phosphopyruvate hydratase (from Yeast), 50% glycerol solution;ENOLASE;EC 4.2.1.11;EC: 4.2.1.11;2-PHOSPHO-D-GLYCERATE HYDRO-LYASE
• CAS NO: 9014-08-8
• Molecular Formula: NULL
• Molecular Weight: 0
• EINECS: 232-754-3
• Product Categories: enzyme
• Mol File: 9014-08-8.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Storage Temp.: 2-8°C
• CAS DataBase Reference: ENOLASE(CAS DataBase Reference)
• NIST Chemistry Reference: ENOLASE(9014-08-8)
• EPA Substance Registry System: ENOLASE(9014-08-8)

Safety Data

• Hazard Codes: B
• WGK Germany: 3
• Hazardous Substances Data: 9014-08-8(Hazardous Substances Data)

Usage

Uses

The enzyme from Sigma has been used as an antigen during ELISA. the study used human granulocyte proteins to identify and characterize autoantibodies against catalase and α-enolase in patients with primary sclerosing cholangitis. It has been used to study temperature- and denaturant-induced yeast enolase denaturation using fourier transform infrared spectroscopy. It has also been used along with other proteins to study gradient chromatofocusing-mass spectrometry; a new technique for protein analysis.

Definition

An enzyme active in glycolysis that catalyzes the conversion of 2-phosphoglyceric acid to the phosphorylated enol of pyruvic acid.

General Description

Enolase (2-phosphoglycerate hydratase) is a cytosolic enzyme. This cytosolic metalloenzyme is encoded by three homologous genes in mammals. Enolase the from baker′s yeast is a homodimer containing two bound Mg2+ ions. The peptide consists of 436 amino acids and contains a single cysteine residue and corresponds to a molecular weight of 93 kDa. Two of the active site components include His191 and Arg414. Enolase is seen in archaea, bacteria and eukaryotes.

Biochem/physiol Actions

Enolase plays a key role in glycolysis and gluconeogenesis. Enolase, found in the nucleus of mammalian cells plays a key role in transcriptional modulation of genes, which participates in morphological transformation and cell proliferation.