Polynucleotide phosphorylase (PNPase) from Bacillus subtilis is an enzyme that plays a crucial role in the degradation and recycling of RNA within the cell. It is a homotetramer, composed of four identical subunits, each containing a catalytic site. PNPase is involved in the breakdown of RNA into its constituent nucleotides, which can then be reused for the synthesis of new RNA molecules. This enzyme exhibits both exonuclease and endonuclease activities, allowing it to degrade RNA from both ends and at internal sites. The enzyme's function is essential for maintaining cellular RNA homeostasis and is particularly important during stress conditions, such as nutrient limitation, where efficient RNA degradation and recycling are required for survival. PNPase from Bacillus subtilis has been extensively studied for its potential applications in biotechnology and molecular biology, including its use in RNA sequencing and gene expression analysis.
The CAS Registry Mumber 9014-12-4 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,1 and 4 respectively; the second part has 2 digits, 1 and 2 respectively. Calculate Digit Verification of CAS Registry Number 9014-12: (6*9)+(5*0)+(4*1)+(3*4)+(2*1)+(1*2)=74 74 % 10 = 4 So 9014-12-4 is a valid CAS Registry Number.
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