9014-25-9

Basic information

• Product Name: TRNA, BAKER'S YEAST
• Synonyms: RIBONUCLEIC ACID, TRANSFER FROM BAKERS*Y EAST;RIBONUCLEIC ACID TRANSFER TYPE XII FROM RABBIT LIVE;RIBONUCLEIC ACID TRANSFER TYPE X-SA*FROM BAKERS YEA;RIBONUCLEIC ACID, TRANSFER FROM RABBIT L IVER;RIBONUCLEIC ACID TRANSFER TYPE XXI*FROM ESCHERICHIA;RIBONUCLEIC ACID, TRANSFER TYPE XI*FROM BOVINE LIVE;RIBONUCLEIC ACID, TRANSFER-MOLECULAR*BIO LOGY GRADE;RIBONUCLEIC ACID TRANSFER TYPE X*FROM BA KERS YEAST
• CAS NO: 9014-25-9
• Molecular Weight: 0
• EINECS: 277-256-7
• Mol File: 9014-25-9.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Storage Temp.: −20°C
• Solubility: H2O: 2 mg/mL, clear, colorless
• CAS DataBase Reference: TRNA, BAKER'S YEAST(CAS DataBase Reference)
• NIST Chemistry Reference: TRNA, BAKER'S YEAST(9014-25-9)
• EPA Substance Registry System: TRNA, BAKER'S YEAST(9014-25-9)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 9014-25-9(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 9014-25-9 differently. You can refer to the following data:
1. Ribonucleic acid, transfer from Escherichia coli, Type XX, Strain W may be used as a starting material for the purification of specific amino acyl-tRNA species by methods such as liquid column chromatography or high-performance liquid chromatography (HPLC).
2. Suitable for use as a carrier in nucleic acid purification and precipitation.Ribonucleic acid, transfer from baker′s yeast (S. cerevisiae) has been used:in carrier solution as a part of control used for reverse transcriptase – quantitative polymerase reaction (RT-qPCR) assayas a component of prehybridization(4) and hybridization buffer in single-label in situ hybridizationas a carrier to enhance recovery of RNA from small numbers of cells
3. Suitable for use as a carrier in nucleic acid purification and precipitation.

General Description

Transfer RNA (tRNA) is isolated from baker′s yeast by phenol-chloroform extraction and ethanol precipitation. tRNAs are approximately 80 nucleotides long RNA molecules with a cloverleaf shaped secondary structure and a tertiary L-shaped structure.

Biochem/physiol Actions

Transfer RNAs (tRNAs) play an important role in translation. It is responsible for the addition of amino acids to ribosome for peptide chain formation. The tRNA contains an anticodon region for mRNA base pairing and two attachment regions: for amino acid binding and tRNA synthetase recognition. The ribosomal RNA facilitates the movement of tRNA along the mRNA.