9015-85-4
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Basic information
- Product Name: T4 DNA LIGASE
- Synonyms: T4 DNA LIGASE;RNA LIGASE, T4 PAHGE;DNA LIGASE;DNA LIGASE NAD-DEPENDENT;DNA LIGASE T4;DNA LIGASE, THERMOSTABLE;EC 6.5.1.1;EC 6.5.1.2
- CAS NO:9015-85-4
- Molecular Formula:
- Molecular Weight: 0
- EINECS: 232-770-0
- Product Categories: Enzyme
- Mol File: 9015-85-4.mol
- Article Data: 0
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Chemical Properties
- Melting Point: N/A
- Boiling Point: N/A
- Flash Point: N/A
- Appearance: /buffered aqueous glycerol solution
- Density: N/A
- Refractive Index: N/A
- Storage Temp.: −20°C
- Solubility: N/A
- CAS DataBase Reference: T4 DNA LIGASE(CAS DataBase Reference)
- NIST Chemistry Reference: T4 DNA LIGASE(9015-85-4)
- EPA Substance Registry System: T4 DNA LIGASE(9015-85-4)
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Safety Data
- Hazard Codes: N/A
- Statements: N/A
- Safety Statements: N/A
- WGK Germany: 3
- RTECS:
- F: 10
- TSCA: Yes
- HazardClass: N/A
- PackingGroup: N/A
- Hazardous Substances Data: 9015-85-4(Hazardous Substances Data)
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9015-85-4 Usage
Uses
Forms an energy dependent phosphodiester linkage between the termini of adjacent polynucleotides of duplex DNA. For ligation of cloning vector and restriction insert fragmentsGeneral Description
T4 DNA Ligase catalyzes the formation of phosphodiester bonds between neighboring 3′-hydroxyl- and 5′-phosphate ends in double-stranded DNA. Single-stranded nicks in double-stranded DNA are also closed by T4 DNA Ligase.ContentsT4 DNA Ligase, supplied with 10x concentrated ligation buffer that includes ATP.Check Digit Verification of cas no
The CAS Registry Mumber 9015-85-4 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,1 and 5 respectively; the second part has 2 digits, 8 and 5 respectively.Calculate Digit Verification of CAS Registry Number 9015-85:
(6*9)+(5*0)+(4*1)+(3*5)+(2*8)+(1*5)=94
94 % 10 = 4
So 9015-85-4 is a valid CAS Registry Number.
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T4 DNA LIGASE
