9025-26-7

Basic information

• Product Name: CATHEPSIN D
• Synonyms: CATHEPSIN D;CATHEPSIN D, BOVINE;CATHEPSIN D, BOVINE KIDNEY;CATHEPSIN D, BOVINE SPLEEN;CATHEPSIN D, HUMAN;CATHEPSIN D, HUMAN LIVER;CATHEPSIN D, HUMAN SPLEEN;EC 3.4.22.5
• CAS NO: 9025-26-7
• Molecular Formula: NULL
• Molecular Weight: 0
• Mol File: 9025-26-7.mol
• Article Data: 0

Chemical Properties

• Appearance: white/lyophilized powder
• Storage Temp.: −20°C
• Water Solubility: Reconstitute in water at 1µg/µl
• CAS DataBase Reference: CATHEPSIN D(CAS DataBase Reference)
• NIST Chemistry Reference: CATHEPSIN D(9025-26-7)
• EPA Substance Registry System: CATHEPSIN D(9025-26-7)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 9025-26-7(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 9025-26-7 differently. You can refer to the following data:
1. Cathepsin D, human liver is useful for inhibitor screening, kinetic studies, and cleavage of substrates in vitro or in tissue culture. It contains the mannose-6-phosphate lysosomal localization signal that targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. It is a useful prognostic indicator for breast cancer.
2. Cathepsin D from human liver has been used:in β-secretase activity assayfor enzymatic degradation of amyloid β 1-42in microinjection of human foreskin fibroblasts

General Description

Cathepsin D is an aspartic protease, which is located in lysosomes. It is involved in protein catabolism and maintains hormone and antigen processing. Cathepsin D is implicated in neoplasia and neurodegenerative changes. It regulates lysosomal proteolysis and endogenous fibrinolysis.

Biochem/physiol Actions

Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.

Purification Methods

Cathepsin D is purified on a CM column after (NH4)2SO4 fractionation and dialysis, then starch-gel electrophoresis and by ultracentrifugal analysis. Finally chromatograph on a DEAE column [Press et al. Biochem J 74 501 1960].