9025-35-8

Basic information

• Product Name: α-galactosidase
• Synonyms: ALPHA-GALACTOSIDASE;ALPHA-GALACTOSIDASE, POSITIONALLY SPECIFIC;ALPHA-D-GALACTOSIDE GALACTOHYDROLASE;ALPHA1,3,6-D-GALACTOSIDE GALACTOHYDROLASE;ALPHA1-3,6-GALACTOSIDASE;MELIBIASE;EC 3.2.1.22;a1-3,6-Galactosidase, Recombinant, E. coli
• CAS NO: 9025-35-8
• Molecular Weight: 0
• EINECS: 232-792-0
• Product Categories: 3.2.x.x Glycosidases;3.x.x.x Hydrolases;Application Index;Carbohydrate and PTM AnalysisApplication Index;Carbohydrate hydrolysis&PTM analysis;Carbohydrate HydrolysisGlycobiology;Carbohydrate-active Enzymes;ExoglycosidasesApplication Index;Organic Synthesis and Immobilized Enzymes;Organic SynthesisEnzyme Class Index
• Mol File: 9025-35-8.mol
• Article Data: 0

Chemical Properties

• Appearance: /buffered aqueous solution
• Storage Temp.: 2-8°C
• CAS DataBase Reference: α-galactosidase(CAS DataBase Reference)
• NIST Chemistry Reference: α-galactosidase(9025-35-8)
• EPA Substance Registry System: α-galactosidase(9025-35-8)

Safety Data

• Hazard Codes: Xi
• Statements: 36/37/38
• Safety Statements: 36/37-26
• WGK Germany: 3
• Hazardous Substances Data: 9025-35-8(Hazardous Substances Data)

Usage

Chemical Properties

Derived from Mortierella vinaceae var. raffinoseutilizer.

Uses

Different sources of media describe the Uses of 9025-35-8 differently. You can refer to the following data:
1. Alkaline α-Galactosidase I was used to assay enzyme activity with 2 mmp-nitrophenyl-α-d-galactoside as substrate at pH 6.5 to compare with the enzyme activity of α-Gal A isolated and purified from Sf-9 insect cells infected with a recombinant baculovirus encoding normal α-Gal A gene.
2. α-Galactosidase from green coffee beans has been used to assess the specificity of anti- α-Galactosidase antibodies. It has also been used in inhibition assay to assess N-guanidino derivatives of 1,5-dideoxy-1,5-imino-d-xylitol activity.

General Description

Alkaline α-Galactosidase I is a glycoside hydrolase, separated from the extracts of melon fruits. The activity of this enzyme is found to increase during the early stages of ovary development and fruit set.

Biochem/physiol Actions

Cleaves α(1→3)- and α(1→6)-linked, non-reducing terminal galactose from complex carbohydrates and glycoproteins. It is particularly efficient for removing α-linked galactose under conditions where the pH must be neutral or above, for example, with live cells.