9026-12-4

Basic information

• Product Name: RIBONUCLEASE T1
• Synonyms: RIBONUCLEASE T;RIBONUCLEASE T1;RIBONUCLEASE T1, ASPERGILLUS ORYZAE;RIBONUCLEATE 3'-GUANYLO-OLIGONUCLEOTIDOHYDROLASE;RNASE A AND T1 MIX;RNASE A + T1 MIX;RNASE A/T1 MIX;RNASE T
• CAS NO: 9026-12-4
• Molecular Weight: 0
• EINECS: 232-809-1
• Mol File: 9026-12-4.mol
• Article Data: 0

Chemical Properties

• Appearance: /ammonium sulfate suspension
• Storage Temp.: 2-8°C
• CAS DataBase Reference: RIBONUCLEASE T1(CAS DataBase Reference)
• NIST Chemistry Reference: RIBONUCLEASE T1(9026-12-4)
• EPA Substance Registry System: RIBONUCLEASE T1(9026-12-4)

Safety Data

• WGK Germany: 3
• RTECS: RF0753000
• F: 10
• Hazardous Substances Data: 9026-12-4(Hazardous Substances Data)

Usage

Uses

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies .

Biochem/physiol Actions

Ribonuclease T1 (RNase T1) from Aspergillus oryzae is an endoribonuclease that hydrolyzes after G residues. Cleavage occurs between the 3′-phosphate group of a guanidine ribonucleotide and 5′-hydroxyl of the adjacent nucleotide. The initial product is a 2′:3′ cyclic phosphate nucleoside that is hydrolyzed to the corresponding 3′-nucleoside phosphate. It differs from Pancreatic RNase in that it attacks the guanine sites specifically to yield 3′-GMP and oligonucleotides with a 3′-GMP terminal group.