3-Phospho-D-glycerate carboxylyase[dimerizing], also known as D-ribulose 1,5-diphosphate carboxylase, is a 557 kDa hexadecamer enzyme composed of eight heavy chains with a molecular weight of approximately 56 kDa and eight light chains of molecular weight 14 kDa. Each molecule contains one magnesium ion. It has a pH optimum of around 7.9 and a KM for CO2 of approximately 0.45 mM. Ribulose diphosphate becomes inhibitory at concentrations exceeding 0.7 mM, while orthophosphate and ammonium sulfate act as competitive inhibitors, and 3-phosphoglycerate is a noncompetitive inhibitor.
9027-23-0
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Basic information
- Product Name: 3-PHOSPHO-D-GLYCERATE CARBOXYLYASE[DIMERIZING]
- Synonyms: EC 4.1.1.39;D-RIBULOSE 1,5-DIPHOSPHATE CARBOXYLASE;3-PHOSPHO-D-GLYCERATE CARBOXYLYASE[DIMERIZING];D-ribulose 1,5-diphosphate carboxylase*from spina;D-RIBULOSE 1,5-DIPHOSPHATE CARBOXYLASE*F ROM SPINACH;rubisco
- CAS NO:9027-23-0
- Molecular Formula:
- Molecular Weight: 0
- EINECS: N/A
- Product Categories: N/A
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Chemical Properties
- Melting Point: N/A
- Boiling Point: N/A
- Flash Point: N/A
- Appearance: /partially purified powder
- Density: N/A
- Refractive Index: N/A
- Storage Temp.: −20°C
- Solubility: N/A
- CAS DataBase Reference: 3-PHOSPHO-D-GLYCERATE CARBOXYLYASE[DIMERIZING](CAS DataBase Reference)
- NIST Chemistry Reference: 3-PHOSPHO-D-GLYCERATE CARBOXYLYASE[DIMERIZING](9027-23-0)
- EPA Substance Registry System: 3-PHOSPHO-D-GLYCERATE CARBOXYLYASE[DIMERIZING](9027-23-0)
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Safety Data
- Hazard Codes: N/A
- Statements: N/A
- Safety Statements: N/A
- WGK Germany: 3
- RTECS:
- HazardClass: N/A
- PackingGroup: N/A
- Hazardous Substances Data: 9027-23-0(Hazardous Substances Data)
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9027-23-0 Usage
Uses
Used in Enzyme Kinetic Studies:3-Phospho-D-glycerate carboxylyase[dimerizing] is used as a test protein in enzyme kinetic studies to investigate its catalytic properties, pH optimum, and substrate inhibition.
Used in Pepsin Digestion Studies:
In the food industry, 3-Phospho-D-glycerate carboxylyase[dimerizing] has been used as a test protein in pepsin digestion studies to understand the enzyme's stability and susceptibility to proteolysis.
Used in Human Intestinal Epithelial Cell Line Studies:
3-Phospho-D-glycerate carboxylyase[dimerizing] is used as an innocuous or non-hazardous protein sample to test its effect on human intestinal epithelial cell lines, providing insights into its potential role in human health and nutrition.
Used in Isothermal Titration Calorimetry (ITC) and Radiolabeled Binding Assays:
In the field of biochemistry, 3-Phospho-D-glycerate carboxylyase[dimerizing] is used in isothermal titration calorimetry (ITC) and radiolabeled binding assays with abscisic acid to study the enzyme's interactions with other molecules and its binding properties.
Biochem/physiol Actions
D-Ribulose 1,5-Diphosphate Carboxylase (RUBISCO) depends on Rubisco activase and chaperones for activation. It participates in plant photorespiration events by catalyzing the carboxylation and oxygenation of ribulose-1,5-bisphosphate. Abscisic acid inhibits the carboxylation activity of Rubisco.Check Digit Verification of cas no
The CAS Registry Mumber 9027-23-0 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,2 and 7 respectively; the second part has 2 digits, 2 and 3 respectively.Calculate Digit Verification of CAS Registry Number 9027-23:
(6*9)+(5*0)+(4*2)+(3*7)+(2*2)+(1*3)=90
90 % 10 = 0
So 9027-23-0 is a valid CAS Registry Number.
9027-23-0Upstream product
9027-23-0Downstream Products
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3-PHOSPHO-D-GLYCERATE CARBOXYLYASE[DIMERIZING]
