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9028-67-5

Choline oxidase is an enzyme that catalyzes the oxidation of choline to betaine and hydrogen peroxide. It is produced by various microorganisms, including Alcaligenes sp. and Sigma. This enzyme plays a crucial role in the metabolism of choline and has been widely studied for its potential applications in various fields.

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  • 9028-67-5 Structure

  • Basic information

    1. Product Name: CHOLINE OXIDASE
    2. Synonyms: EC 1.1.3.17;CHOLINE OXIDASE;CHOLINE OXYGEN 1-OXIDOREDUCTASE;CHOLINE: OXYGEN 1-OXIDOREDUCTASE;choline oxidase from alcaligenes sp.;choline oxidase from arthrobacter*globiformis;Oxidase, choline;CHOLINE OXIDASE FROM ALCALIGENESSPECIES
    3. CAS NO:9028-67-5
    4. Molecular Formula: NULL
    5. Molecular Weight: 0
    6. EINECS: 232-840-0
    7. Product Categories: N/A
    8. Mol File: 9028-67-5.mol
    9. Article Data: 0

  • Chemical Properties

    1. Melting Point: N/A
    2. Boiling Point: N/A
    3. Flash Point: N/A
    4. Appearance: yellow/powder
    5. Density: N/A
    6. Refractive Index: N/A
    7. Storage Temp.: −20°C
    8. Solubility: N/A
    9. CAS DataBase Reference: CHOLINE OXIDASE(CAS DataBase Reference)
    10. NIST Chemistry Reference: CHOLINE OXIDASE(9028-67-5)
    11. EPA Substance Registry System: CHOLINE OXIDASE(9028-67-5)

  • Safety Data

    1. Hazard Codes: N/A
    2. Statements: N/A
    3. Safety Statements: 22-24/25
    4. WGK Germany: 3
    5. RTECS:
    6. F: 10-21
    7. HazardClass: N/A
    8. PackingGroup: N/A
    9. Hazardous Substances Data: 9028-67-5(Hazardous Substances Data)

9028-67-5 Usage

Uses

Used in Biosensor Applications:
Choline oxidase from Alcaligenes sp. is used as a key component in the construction of choline biosensors. These biosensors are designed to measure cholinesterase inhibitory activities, which are essential for understanding the effects of certain drugs and toxins on the nervous system.
Used in Spectroscopy Studies:
Choline oxidase from Alcaligenes sp. is also used in spectroscopic studies to investigate the photoreaction of choline oxidase with covalently bound flavin. This research helps to better understand the enzyme's structure and function, as well as its potential applications in various fields.
Used in Enzymatic Determination of Choline:
Choline oxidase from Sigma is used in the enzymatic determination of choline in milk using a flow injection analysis (FIA) system with potentiometric detection. This method allows for accurate and efficient measurement of choline levels in milk, which is important for assessing the nutritional value and quality of dairy products.

Biochem/physiol Actions

Choline oxidase catalyzes the four-electron oxidation of choline to glycine-betaine, with betaine-aldehyde as intermediate and molecular oxygen as primary electron acceptor. The enzyme can also accept betaine-aldehyde as a substrate. This allows the investigation of the reaction mechanism for the conversion of choline to the aldehyde intermediate and of betaine-aldehyde to glycine-betaine. The enzyme is a flavoprotein with a molecular weight of approximately 83,000 Da according to gel filtration or approximately 71,000 Da according to SDS gel electrophoresis. The optimum pH is found to be around pH 7.5 and the isoelectric point (pI) around pH 4.5.

Check Digit Verification of cas no

The CAS Registry Mumber 9028-67-5 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,2 and 8 respectively; the second part has 2 digits, 6 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 9028-67:
(6*9)+(5*0)+(4*2)+(3*8)+(2*6)+(1*7)=105
105 % 10 = 5
So 9028-67-5 is a valid CAS Registry Number.

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