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Usage

Uses

Used in Pharmaceutical Industry:
Trypsin inhibitor is used as a specific reversible inhibitor of trypsin and a potent inhibitor of human factor XIIa. It is employed to produce denatured, oxidized, and deglycosylated ovomucoid, which is used to stimulate patient-derived cell cultures for mapping T cell epitopes and to treat nuclei for flow cytometric analysis.
Used in Research Applications:
In Holtfreter's solution, trypsin inhibitor is used for the dissociation of planarians into cells, aiding in various research studies.
Used in Cell Culture Applications:
This product has been tested for cell culture applications, where trypsin has been used in studies to assess the potential application in animal cell culture of an alkaline protease from a non-toxigenic mangrove isolate of Vibrio sp. V26. Additionally, trypsin has been used in a study to improve the detection of fungi in eosinophilic mucin.

Biochem/physiol Actions

This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.