9035-81-8

Basic information

• Product Name: Trypsin inhibitor
• Synonyms: TRYPSIN INHIBITOR CHICKEN;TRYPSIN INHIBITOR, LIMA BEAN;TRYPSIN INHIBITOR LIMA BEANS;LIMA BEAN TRYPSIN INHIBITOR;KUNITZ TRYPSIN INHIBITOR;APROTINIUM;trypsin inhibitor 1% sterile solution;trypsin inhibitor from lima beans
• CAS NO: 9035-81-8
• Molecular Formula: NULL
• Molecular Weight: ＜50000
• EINECS: 232-906-9
• Product Categories: Miscellaneous Biochemicals;DissociationIslet Stem Cell Biology;DissociationPrimary Human Cell Culture;Endothelial Cells;Human Umbilical Vein Endothelial Cell (HUVEC) Culture Companion Products and Supplements;Islet Stem Cell Isolation;Neural Stem Cell Biology;Neural Stem Cell Isolation/Expansion;Chymotrypsin, alpha-Enzyme Inhibitors by Enzyme;Chymotrypsin, α-Enzyme Inhibitors by Enzyme;Trypsin Inhibitors;A to;Protease Inhibitor Specificity Index;Protease Inhibitors;R to;inhibitor
• Mol File: 9035-81-8.mol
• Article Data: 0

Chemical Properties

• Appearance: /solution
• Storage Temp.: 2-8°C
• Solubility: H2O: >10 mg/mL
• Water Solubility: Soluble in water (10 mg/ml), phosphate buffers (10 mg/ml), and balanced salt solutions (1 mg/ml).
• CAS DataBase Reference: Trypsin inhibitor(CAS DataBase Reference)
• NIST Chemistry Reference: Trypsin inhibitor(9035-81-8)
• EPA Substance Registry System: Trypsin inhibitor(9035-81-8)

Safety Data

• Hazard Codes: Xn
• Statements: 42/43
• Safety Statements: 22-36/37-45-23
• WGK Germany: 3
• F: 10-21
• Hazardous Substances Data: 9035-81-8(Hazardous Substances Data)

Usage

Uses

Different sources of media describe the Uses of 9035-81-8 differently. You can refer to the following data:
1. Trypsin Inhibitor, Corn is a specific reversible inhibitor of trypsin and potent inhibitor of human factor XIIa.
2. Trypsin inhibitor from chicken egg white has been used:to produce denatured, oxidized and deglycosylated ovomucoid and used to stimulate patient derived cell cultures for mapping T cell epitopesto treat nuclei for flow cytometric analysisin Holtfreter′s solution, used for the dissociation of planarians into cells
3. This product has been tested for cell culture applications. Trypsin has been used in a study to assess the potential application in animal cell culture of an alkaline protease from a ton-toxigenic mangrove isolate of Vibri sp. V26. Trypsin has also been used in a study to improve the detection of fungi in eosinophilic mucin.

General Description

Ovomucoid or trypsin inhibitor is an abundant protein in most avian egg whites. The hen′s egg protein is composed of about 186 amino acid residues, and is highly glycosylated. Three tandem domains are each homologous with pancreatic trypsin inhibitor. It is highly immunogenic, and probably accounts for most cases of egg allergy.

Biochem/physiol Actions

This inhibitor acts against trypsin, and chymotrypsin and plasmin to a lesser extent. It will also inhibit proteases with mechanisms similar to trypsin, plasma kallikrein and coagulation Factor X. The trypsin inhibitor will not act against metalloproteases, tissue-baseed kallikrein, acid proteases, or thio proteases. This inhibitor acts by forming a 1:1 stoichiometric complex with the protease active site, and then cleaving a single arginine-isoleucine bond on the inhibitor. The inhibition is both reversible and pH dependent.