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9048-46-8

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9048-46-8 Usage

Description

Different sources of media describe the Description of 9048-46-8 differently. You can refer to the following data:
1. Bovine serum albumin is a protein which has a key role in regulating blood colloidal osmotic pressure. A water-soluble protein composed of 583 amino acids, BSA has a calculated molecular weight of 66,430 Daltons. Six α-helices form three homologous domains of BSA. Depending on pH, BSA undergoes reversible conformational isomerization. The native structure of the protein becomes reactive and flexible on heating.This BSA product is specifically evaluated and tested for the following properties:Very low protease content / protease-freeVery low fatty acid content / fatty acid-freeProtease-free BSA is especially useful for applications like:Enzyme assaysProtein-based assaysProtease-sensitive techniques that include:a. Enzyme immunoassay (EIA)b. Nucleic acid hybridizationc. Radioimmunoassay (RIA)
2. Acetylated BSA may be used as a positive control for ELISA or WB when employing an acetyl-lysine monoclonal or polyclonal antibody. Cayman’s Acetyl Lysine Monoclonal Antibody (Clone 7F8) can detect less than 1 ng of this positive control by immunoblotting. Dilutions from a 1 mg/ml stock solution and 2X Laemmli Buffer may be prepared for WB use. For example, dissolve the 1 mg lyophilized protein in 1 ml of purified water (aliquot and freeze for later, use as needed), then make ten-fold dilutions until the desired concentration range is obtained. Finally dilute the product 1:1 in 2X Laemmli Buffer. Incubate the prepared sample for five minutes in boiling water followed by five minutes on ice prior to loading gels.Histone subunit modifications such as lysine acetylation are regulated by the activity of histone acetyltransferases (HATs) and histone deacetylases (HDACs). Epigenetic modifications such as protein acetylation directly influence cellular genetic programs including those contributing to cancer cell viability.

Chemical Properties

Crystalline powder

Uses

Different sources of media describe the Uses of 9048-46-8 differently. You can refer to the following data:
1. Suitable for cell culture media supplement.Bovine serum albumin (BSA) Fraction V has uses in many applications such as stabilizing proteins in solution, blocking non-specific binding in immuno-assays, and as a protein standard in various protein assays.Stabilizer for proteins and enzymes in solutionBlocking agent for immuno-assays (ie, western blot, IHC, IF)Minimizes non-specific antibody bindingIgG- and protease-free50 g of solid powderBovine serum albumin (BSA) is commonly used as a stabilizing agent for proteins and enzymes, including dilute solutions of antibody. It is also used as a blocking agent to reduce non-specific antibody binding in immuno-detection procedures such as western blotting, immunofluorescence, and IHC. It is also suitable for use in protease sensitive assays such as RIA and EIA, as well as nucleic acid hybridization. This BSA is IgG- and protease-free.
2. Select the right albumin for your application with the Albumin Selection GuideBSA has various uses in molecular biology applications, such as:As an added component in solutions of enzymes or of other proteins, to prevent adhesion of the enzyme or other protein of interest to the reaction tube surface or to pipette surfacesAs an inert enzyme or protein stabilizer during incubation, to allow the enzyme or protein of interest to function properly
3. Protease-sensitive Immunoassays such as RIA, EIA, Fluorescent and Chemiluminescent, Protein Standard, Diluent, Protein, Conjugate or Enzyme Stabilizer. Bovine Serum Albumin is applicable for numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry. Frequently used as a blocking reagent; particularly useful with casein-sensitive antibodies, such as phospho-specific antibodies. Also used as a nutrient in cell and microbial culture. In restriction digests, BSA is used to stabilize some enzymes during digestion of DNA and to prevent adhesion of the enzyme to reaction tubes and other vessels. Commonly used to determine the quantity of other proteins, by comparing an unknown quantity of protein to known amounts of BSA. Hybridization, Selected Cell Culture applications

Definition

The word bovine means “cow,” and bovine serum albumin (BSA) is a protein that comes from cows. In particular, BSA is a type of protein called albumin, which is found in large amounts in the blood of cows. Blood is a mixture of red blood cells, which is why blood looks red, and many different types of proteins that are dissolved in water. If the cells in blood were removed, you would be left with a clear liquid, called serum. Thus, BSA is the albumin protein that is found in the blood of cows. In the blood, the job of albumin is to carry other proteins from place to place.

Application

Bovine Serum Albumin has been used:as a constituent of medium used for the differentiation of human pluripotent stem cellsin enzyme-linked immunosorbent assay (ELISA) and sandwich ELISAto permeabilize fetal cortical cellsThis Sigma A7030 BSA has specifically been used as a component of blocking buffer in a peptide-based ELISA assay for analysis of COVID-19 / SARS-CoV-2 samples (Poh, C.K. et al., Nat. Commun., 11(1), 2806 (2020)).

General Description

Bovine serum albumin (BSA) has an important role in the regulation of blood colloidal osmotic pressure. BSA is the major protein in bovine serum, and is composed of 583 amino acids with a calculated molecular weight of 66,430 Daltons. BSA is used in many different applications and fields, such as:Protein quantitation (as a standard)Immunochemistry (as a blocking agent)Cell culture (as a nutrient)This particular BSA is specifically evaluated and tested to have very low protease content / for protease-free status. Protease-free BSA may be used in applications like:Enzyme assaysProtein-based assaysProtease-sensitive techniques that include:a) Enzyme immunoassay (EIA)b) Nucleic acid hybridizationc) Radioimmunoassay (RIA)

Biological Activity

Bovine Serum Albumin is a BSA (bovine serum albumin) is a widely used blocking reagent for use in immunohistochemistry (IHC), Immunocytochemistry (ICC), ELISAs and Western blotting. Used to block non-specific binding of antibodies. Can also be used as a protein concentration standard in Bradford assay for protein quantification. BSA is also used in cell culture.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Check Digit Verification of cas no

The CAS Registry Mumber 9048-46-8 includes 7 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 4 digits, 9,0,4 and 8 respectively; the second part has 2 digits, 4 and 6 respectively.
Calculate Digit Verification of CAS Registry Number 9048-46:
(6*9)+(5*0)+(4*4)+(3*8)+(2*4)+(1*6)=108
108 % 10 = 8
So 9048-46-8 is a valid CAS Registry Number.

9048-46-8 Well-known Company Product Price

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  • (NIST927E)  Bovine Serum Albumin (7% solution)  NIST® SRM® 927e

  • 9048-46-8

  • NIST927E

  • 13,945.23CNY

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9048-46-8SDS

SAFETY DATA SHEETS

According to Globally Harmonized System of Classification and Labelling of Chemicals (GHS) - Sixth revised edition

Version: 1.0

Creation Date: Aug 14, 2017

Revision Date: Aug 14, 2017

1.Identification

1.1 GHS Product identifier

Product name Serum albumin

1.2 Other means of identification

Product number -
Other names Albumin

1.3 Recommended use of the chemical and restrictions on use

Identified uses For industry use only.
Uses advised against no data available

1.4 Supplier's details

1.5 Emergency phone number

Emergency phone number -
Service hours Monday to Friday, 9am-5pm (Standard time zone: UTC/GMT +8 hours).

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