9075-68-7

Basic information

• Product Name: PULLULANASE
• Synonyms: pullulanase from bacillus sp.;pullulanase from klebsiella pneumoniae;PULLULANASE FROM BACILLUS SP., POWDER, ~ 1 U/MG;PULLULANASE FROM BACILLUS*ACIDOPULLULYTI;pullulanase from bacillus acidopullulyticus;PULLULANASE SUSPENSION, FROM KLEBSIELLA PNEUMONIAE;Amylopectin 6-gluconohydrolase, Limit dextrinase;PromozymeTM 400L, Pullulan 6-glucano-hydrolase
• CAS NO: 9075-68-7
• Molecular Formula: NULL
• Molecular Weight: 0
• EINECS: 232-983-9
• Mol File: 9075-68-7.mol
• Article Data: 0

Chemical Properties

• Appearance: /lyophilized powder
• Density: 1.25 g/mL at 25 °C(lit.)
• Vapor Pressure: 0Pa at 25℃
• Storage Temp.: 2-8°C
• Water Solubility: 100g/L at 25℃
• CAS DataBase Reference: PULLULANASE(CAS DataBase Reference)
• NIST Chemistry Reference: PULLULANASE(9075-68-7)
• EPA Substance Registry System: PULLULANASE(9075-68-7)

Safety Data

• Safety Statements: 22-24/25
• WGK Germany: 3
• Hazardous Substances Data: 9075-68-7(Hazardous Substances Data)

Usage

Description

As an an amylolytic exoenzyme, Pullulanase is one of the most essential endo-type enzyme that degrades pullulan. It is found in plants and microorganisms, in which it is synthesized as an extracellular, cell surface-anchored lipoprotein by the Gram-negative bacterium such as Bacillus brevis, genus Klebsiella. Type I pullulanases specifically cleaves α-1,6-glucosidic linkages in polysaccharides, such as starch, amylopectin, glycogen, dextrin, pullulan and related oligosaccharides, whilst type II pullulanases are effective to attack α-1,6-glucosidic linkages. This enzyme is applied on a large scale in glucose and maltose syrup industries, where it is combined with the activities of other enzymes, such as β-amylase and isoamylase to manufacture maltose and oligosaccharides. Besides, it commercially serves as an industrial enzyme preparation in various reactions, such as the production of branched cyclodextrins, where pullulanase can catalyze the condensation reaction. Pullulanase is also applied as a processing aid in grain processing biotechnology for the production of ethanol and sweeteners.

References

https://en.wikipedia.org/wiki/Pullulanase http://www.sigmaaldrich.com/catalog/product/sigma/e2412?lang=en®ion=US https://www.hindawi.com/journals/er/2012/921362/ http://www.amano-enzyme.co.jp/eng/enzyme/15.html http://www.ssjournals.com/index.php/ijbr/article/view/610

Uses

Different sources of media describe the Uses of 9075-68-7 differently. You can refer to the following data:
1. One unit will liberate 1.0 μmole of maltotriose (measured as glucose) from pullulan per min at pH 5.0 at 25°C.
2. Pullulanase is a glucanase that degrades pullulan. It is produced by the Gram-negative bacterium Klebsiella and is used for starch-debranching as well as to study polysaccharide utilization.

Biochem/physiol Actions

Type I pullulanases specifically hydrolyse α-1,6 linkages, while type II pullulanases are also able to hydrolyse α-1,4 linkages.