90913-08-9Relevant academic research and scientific papers
Mass spectrometric studies of the path of carbon in photosynthesis: Positional isotopic analysis of 13C-labelled C4 to C7 sugar phosphates
MacLeod, John K.,Flanigan, Ian L.,Williams, John F.,Grant Collins
, p. 500 - 508 (2007/10/03)
The (EIMS) electron ionization mass spectrometric fragmentation patterns of the methoxime- and ethoximetrimethylsilyl (TMS) derivatives of C4 to C7 sugars involved as phosphates in the Calvin pathway of photosynthesis in plants were analysed by gas chromatography/EIMS using specifically labelled 13C analogs. In general, most but not all of the major ions in the mass spectra arise from single carbon-carbon bond cleavages of the straight-chain derivatives. The results confirm that GC/MS of the alkoxime-TMS derivatives is a viable method for measuring 163C incorporations at individual carbon atoms in each of the sugar phosphates during photosynthetic experiments with 13CO2. Copyright
Two-bond 13C-13C spin-coupling constants in carbohydrates: New measurements of coupling signs
Zhao, Shikai,Bondo, Gail,Zajicek, Jaroslav,Serianni, Anthony S.
, p. 145 - 152 (2007/10/03)
D-(1,3,6-13C3)Allose (1), (13C)methyl α-D-(1,2-13C2)glucopyranoside (2) and (13C)methyl β-D-(1,2-13C2)glucopyranoside (3) were synthesized and used to establish the signs of their constituent 2J(CCC) or 2J(COC) values (2J(C1,C3) in the α-pyranose of 1 (15), and 2J(C1,CH3) in 2 and 3). Compounds 2, 3 and 15 contain three mutually coupled labeled carbons, thus creating a three-spin system from which crosspeak displacements in 13C-13C COSY-45 spectra were used to determine coupling signs. In all compounds, at least one 3J(CC) value was present as an internal reference: 3J(C2,CH3 in 2 and 3, and 3J(C1,C6) and 3J(C3,C6) in 15. 2J(C1,CH3), in 2 and 3, and 2J(C1,C3) in 15, were found to be negative, thus providing experimental confirmation of the sign predictions made via the projection resultant rule described recently.
