90913-09-0Relevant articles and documents
Mass spectrometric studies of the path of carbon in photosynthesis: Positional isotopic analysis of 13C-labelled C4 to C7 sugar phosphates
MacLeod, John K.,Flanigan, Ian L.,Williams, John F.,Grant Collins
, p. 500 - 508 (2007/10/03)
The (EIMS) electron ionization mass spectrometric fragmentation patterns of the methoxime- and ethoximetrimethylsilyl (TMS) derivatives of C4 to C7 sugars involved as phosphates in the Calvin pathway of photosynthesis in plants were analysed by gas chromatography/EIMS using specifically labelled 13C analogs. In general, most but not all of the major ions in the mass spectra arise from single carbon-carbon bond cleavages of the straight-chain derivatives. The results confirm that GC/MS of the alkoxime-TMS derivatives is a viable method for measuring 163C incorporations at individual carbon atoms in each of the sugar phosphates during photosynthetic experiments with 13CO2. Copyright