92557-80-7

Basic information

• Product Name: 5-Carboxyfluorescein N-succinimidyl ester
• Synonyms: 5-CARBOXYFLUORESCEIN, SUCCINIMIDYL ESTER;5-CARBOXYFLUORESCEIN-NHS;5-CARBOXYFLUORESCEIN-N-HYDROXYSUCCINIMIDE ESTER;5-CARBOXYFLUORESCEIN N-SUCCINIMIDYL ESTER;5(6)-FAM-NHS;1-[[36Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9[9H]xanthen]-5-yl)carbonyl]oxy]-2,5-pyrrolidinedione;carboxyfluorescein-NHS ester;5-FAM SE, 5-CF SE
• CAS NO: 92557-80-7
• Molecular Formula: C25H15NO9
• Molecular Weight: 473.39
• Product Categories: Fluorescent Labels & Indicators;Fluorescein and DerivativesFluorescent Stains;Nucleic Acid Stains For Microscopy;Biochemicals and Reagents;DetectionFluorescent Probes, Labels, Particles and Stains;Fluorescent Labels;Fluorescent Probes, Labels, Particles and Stains;Nucleic Acid Stains;marker
• Mol File: 92557-80-7.mol
• Article Data: 0

Chemical Properties

• Melting Point: >300°C (dec.)
• Boiling Point: 778.7 °C at 760 mmHg
• Flash Point: 424.7 °C
• Appearance: Yellow to Orange Solid
• Density: 1.74g/cm³
• Vapor Pressure: 3.83E-25mmHg at 25°C
• Refractive Index: 1.79
• Storage Temp.: −20°C
• Solubility: Aqueous Base (Slightly), DMSO (Slightly), Methanol (Slightly)
• PKA: 9.31±0.20(Predicted)
• BRN: 7865828
• CAS DataBase Reference: 5-Carboxyfluorescein N-succinimidyl ester(CAS DataBase Reference)
• NIST Chemistry Reference: 5-Carboxyfluorescein N-succinimidyl ester(92557-80-7)
• EPA Substance Registry System: 5-Carboxyfluorescein N-succinimidyl ester(92557-80-7)

Safety Data

• WGK Germany: 3
• F: 8-10-21
• Hazardous Substances Data: 92557-80-7(Hazardous Substances Data)

Usage

Chemical Properties

5-Carboxyfluorescein N-succinimidyl ester is Yellow to Orange Solid

Uses

Different sources of media describe the Uses of 92557-80-7 differently. You can refer to the following data:
1. 5-Carboxyfluorescein N-succinimidyl ester is one of the most popular green fluorescent reagent used for labelling peptides, proteins and nucleotides
2. Amine-reactive derivate of 5-carboxyfluorescein single isomer.
3. 5-Carboxyfluorescein-N-hydroxysuccinimide Ester is one of the most popular green fluorescent probe used for labeling peptides, proteins and nucleotides. It was used as a reagent in the determination of the intracellular pH in bacteria.

InChI:InChI=1/C25H15NO9/c27-13-2-5-17-19(10-13)33-20-11-14(28)3-6-18(20)25(17)16-4-1-12(9-15(16)24(32)34-25)23(31)35-26-21(29)7-8-22(26)30/h1-6,9-11,27-28H,7-8H2