933446-87-8Relevant academic research and scientific papers
Efficient tryptic proteolysis accelerated by laser radiation for peptide mapping in proteome analysis
Yao, Guoping,Deng, Chunhui,Zhang, Xiangmin,Yang, Pengyuan
supporting information; experimental part, p. 8185 - 8189 (2011/02/22)
Back to basics: Coupled with MALDI-TOF MS, laser-assisted proteolysis (see schematic illustration) enabled rapid protein digestion and peptide mapping without the need for enzyme immobilization to increase the efficiency of tryptic digestion. Protein solutions containing trypsin were digested in less than a minute upon irradiation at 808 nm with a laser.
