936327-10-5

Basic information

• Product Name: Aminoallyl-dUTP sodium salt
• Synonyms: AA-dUTP;5-(3-Amino-1-propen-1-yl)-2'-deoxyuridine 5'-(tetrahydrogen triphosphate) sodium salt (1:);AA-dUTP [Aminoallyl dUTP sodium salt] *4 mM in Tris Buffer (pH 7.5)* *CAS 936327-10-5*;5-Aminoallyl 2'-deoxyuridine 5'-triphosphate sodium salt - 100mM aqueous solution
• CAS NO: 936327-10-5
• Molecular Formula: C12H20N3NaO14P3+
• Molecular Weight: 546.209953
• Mol File: 936327-10-5.mol
• Article Data: 0

Chemical Properties

• Appearance: /
• Storage Temp.: ?70°C
• CAS DataBase Reference: Aminoallyl-dUTP sodium salt(CAS DataBase Reference)
• NIST Chemistry Reference: Aminoallyl-dUTP sodium salt(936327-10-5)
• EPA Substance Registry System: Aminoallyl-dUTP sodium salt(936327-10-5)

Safety Data

• WGK Germany: 3
• Hazardous Substances Data: 936327-10-5(Hazardous Substances Data)

Usage

Description

Aminoallyl-dUTP sodium salt is a modified deoxyuridine triphosphate (dUTP) molecule that contains an additional aminoallyl group. This modification allows for the covalent attachment of various functional groups, making it a versatile reagent in molecular biology and biotechnology applications.

Uses

Used in Molecular Biology Research:
Aminoallyl-dUTP sodium salt is used as a reagent for complementary DNA (cDNA) synthesis to incorporate the modified nucleotide into the cDNA strand. This allows for the subsequent attachment of reporter molecules, such as fluorescent dyes or affinity tags, enabling the detection and analysis of specific DNA sequences.
Used in Chromatin Immunoprecipitation (ChIP) Assays:
Aminoallyl-dUTP sodium salt is used as a component in the generation of ChIP probes to label DNA fragments that have been immunoprecipitated with specific antibodies. The incorporation of the modified nucleotide facilitates the detection and quantification of protein-DNA interactions, providing insights into gene regulation and chromatin structure.
Used in Fluorescence In Situ Hybridization (FISH) Techniques:
Aminoallyl-dUTP sodium salt is used as a precursor for the synthesis of fluorescently labeled probes in FISH applications. The modified nucleotide allows for the covalent attachment of fluorescent dyes, enabling the visualization of specific DNA sequences within cells or tissues.
Used in RNA Analysis:
Aminoallyl-dUTP sodium salt is used in the reverse transcription of RNA to generate cDNA probes with attached functional groups. This enables the detection and analysis of specific RNA molecules, providing valuable information on gene expression and regulation.

Biochem/physiol Actions

Aminoallyl-dUTP sodium salt/5-(3-aminoallyl)-2′-deoxyuridine 5′-triphosphate (aa-dUTP) have been used mainly in two-step labeling approach for the synthesis of nucleic acid hybridisation probe.