93939-77-6 Usage
Description
2',3'-Dideoxycytidine 5'-triphosphate lithium salt is a nucleoside triphosphate analog that functions as a chain terminator in DNA replication processes. It is characterized by the absence of a 3'-hydroxyl group, which inhibits the addition of subsequent nucleotides by DNA polymerase, thereby terminating DNA synthesis at the point of incorporation. This unique property makes it a crucial component in the development of nucleic acid sequencing technologies and various molecular biology applications, enhancing the stability and solubility of the compound in solution due to its lithium salt form.
Uses
Used in Molecular Biology Research:
2',3'-Dideoxycytidine 5'-triphosphate lithium salt is used as a substrate in DNA sequencing reactions, specifically for the Sanger sequencing method. Its role as a chain terminator is essential for determining the sequence of nucleotides in a DNA molecule, contributing to the advancement of genetic research and diagnostics.
Used in DNA Sequencing Technologies:
In the field of DNA sequencing, 2',3'-Dideoxycytidine 5'-triphosphate lithium salt serves as a critical component for identifying the order of nucleotides within a DNA strand. Its ability to halt DNA synthesis at specific points allows for the precise determination of DNA sequences, which is fundamental for understanding genetic information and its implications in various biological processes and diseases.
Used in Laboratory Applications:
2',3'-Dideoxycytidine 5'-triphosphate lithium salt is utilized in laboratory settings as a reagent for conducting DNA sequencing experiments. Its increased stability and solubility in solution, attributed to the lithium salt form, make it a reliable and efficient tool for molecular biology research and analysis.
Check Digit Verification of cas no
The CAS Registry Mumber 93939-77-6 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 9,3,9,3 and 9 respectively; the second part has 2 digits, 7 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 93939-77:
(7*9)+(6*3)+(5*9)+(4*3)+(3*9)+(2*7)+(1*7)=186
186 % 10 = 6
So 93939-77-6 is a valid CAS Registry Number.