94236-24-5Relevant articles and documents
Generic Hapten Synthesis, Broad-Specificity Monoclonal Antibodies Preparation, and Ultrasensitive ELISA for Five Antibacterial Synergists in Chicken and Milk
Li, Hongfang,Ma, Shaoqin,Zhang, Xiya,Li, Chenglong,Dong, Baolei,Mujtaba, Mari Ghulam,Wei, Yujie,Liang, Xiao,Yu, Xuezhi,Wen, Kai,Yu, Wenbo,Shen, Jianzhong,Wang, Zhanhui
, p. 11170 - 11179 (2018)
An antibody with broad specificity and principally depending on hapten structure and size is a key reagent for developing a class-selective immunoassay. In the present study, three new generic haptens of antibacterial synergists (ASGs) were proposed using
Conditional glycosylate in eukaryotic cells using a biocompatible chemical inducer of dimerization
Czlapinski, Jennifer L.,Schelle, Michael W.,Miller, Lawrence W.,Laughlin, Scott T.,Kohler, Jennifer J.,Cornish, Virginia W.,Bertozzi, Carolyn R.
supporting information; experimental part, p. 13186 - 13187 (2009/02/06)
Chemical inducers of dimerization (CIDs) are cell-permeable small molecules capable of dimerizing two protein targets. The most widely used CID, the natural product rapamycin and its relatives, is immunosuppressive due to interactions with endogenous targets and thus has limited utility in vivo. Here we report a new biocompatible CID, Tmp-SLF, which dimerizes E. coli DHFR and FKBP and has no endogenous mammalian targets that would lead to unwanted in vivo side effects. We employed Tmp-SLF to modulate gene expression in a yeast three-hybrid assay. Finally, we engineered the Golgi-resident glycosyltransferase FucT7 for tunable control by Tmp-SLF in mammalian cells. Copyright
