946400-30-2Relevant academic research and scientific papers
Synthesis of a new bifunctionalised fluorescent label and physical properties of the bound form on model peptide of troponin C
Hirayama, Tasuku,Iyoshi, Shohei,Taki, Masayasu,Maeda, Yuichiro,Yamamoto, Yukio
, p. 2040 - 2045 (2008/02/12)
A new bifunctional fluorescent label, BRos, was synthesised in order to monitor protein dynamics using fluorescence microscopy, and the photophysical properties were compared with those of bifunctionalised rhodamine, BRho. In a labelling experiment with a model peptide of troponin C, which regulates muscle contraction and relaxation, it was found that BRos was bound to the peptide through two linkages and provided a homogeneous compound, whereas BRho gave a pair of diastereomers having different physical properties in NMR and HPLC analyses. This journal is The Royal Society of Chemistry.
