947510-33-0Relevant academic research and scientific papers
The chemical mechanism of D-1-deoxyxylulose-5-phosphate reductoisomerase from Escherichia coli
Wong, Ursula,Cox, Russell J.
, p. 4926 - 4929 (2007)
(Chemical Equation Presented) Find the way: 3-[2H]- and 4-[ 2H]-labeled 1-deoxyxylulose-5-phosphate were synthesized and used to investigate the chemical mechanism of 1-deoxyxylulose-5-phosphate reductoisomerase (DXR) from E. coli. The observation of inverse secondary kinetic isotope effects for both labeled substrates indicates that DXR uses a retro-aldol/aldol mechanism in which the recombination reaction is the rate-limiting step (see scheme).
