Trityl-Phe-ONP, also known as 9-fluorenylmethyloxycarbonyl-L-phenylalanine p-nitrophenyl ester, is a synthetic chemical compound commonly used in peptide synthesis and as a substrate for protease enzymes. It consists of a trityl group, a phenylalanine amino acid, and a p-nitrophenol (ONP) group. The trityl group serves as a protecting group for the peptide's amino terminus, while the ONP group acts as a chromogenic leaving group, allowing for the colorimetric detection of protease activity. Upon enzymatic cleavage, the p-nitrophenol is released, and its absorbance at 410 nm can be measured, providing a quantitative assessment of enzyme activity. Trityl-Phe-ONP is a valuable tool in biochemical research and drug discovery, particularly for studying the kinetics and specificity of proteases.
The CAS Registry Mumber 96980-02-8 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 9,6,9,8 and 0 respectively; the second part has 2 digits, 0 and 2 respectively. Calculate Digit Verification of CAS Registry Number 96980-02: (7*9)+(6*6)+(5*9)+(4*8)+(3*0)+(2*0)+(1*2)=178 178 % 10 = 8 So 96980-02-8 is a valid CAS Registry Number.