98186-59-5Relevant academic research and scientific papers
Synthesis and characterization of new 3'-3' linked oligodeoxyribonucleotides for alternate strand triple helix formation
De Napoli, Lorenzo,Di Fabio, Giovanni,Messere, Anna,Montesarchio, Daniela,Musumeci, Domenica,Piccialli, Gennaro
, p. 9899 - 9914 (1999)
Protected forms of 1,2,3-propanetriol and cis, cis-1,3,5- cyclohexanetriol were incorporated onto solid supports which were exploited in the solid phase synthesis of 3'-3' linked oligodeoxyribonucleotides (ODNs), involving only nucleoside 3'-phosphoramidites as building blocks. UV thermal denaturation analysis showed the ability of ODNs with this inversion of polarity motif to cooperatively hybridize with duplexes of the type 5'- (Pu)(m)(Py)(n)-3' in an alternate strand recognition approach.
Lipid-modified oligonucleotide conjugates: Insights into gene silencing, interaction with model membranes and cellular uptake mechanisms
Ugarte-Uribe, Bego?a,Grijalvo, Santiago,Pertí?ez, Samuel Nú?ez,Busto, Jon V.,Martín, César,Alagia, Adele,Go?i, Félix M.,Eritja, Ramón,Alkorta, Itziar
supporting information, p. 175 - 186 (2016/12/23)
The ability of oligonucleotides to silence specific genes or inhibit the biological activity of specific proteins has generated great interest in their use as research tools and therapeutic agents. Unfortunately, their biological applications meet the limitation of their poor cellular accessibility. Developing an appropriate delivery system for oligonucleotides is essential to achieve their efficient cellular uptake. In the present work a series of phosphorothioate lipid–oligonucleotide hybrids were synthesized introducing covalently single or double lipid tails at both 3′- and 5′-termini of an antisense oligonucleotide. Gene transfections in cultured cells showed antisense luciferase inhibition without the use of a transfecting agent for conjugates modified with the double-lipid tail at 5′-termini. The effect of the double lipid-tailed modification was further studied in detail in several model membrane systems as well as in cellular uptake experiments. During these studies the spontaneous formation of self-assembled microstructures is clearly observed. Lipidation allowed the efficient incorporation of the oligonucleotide in HeLa cells by a macropinocytosis mechanism without causing cytotoxicity in cells or altering the binding properties of the oligonucleotide conjugates. In addition, both single- and double-tailed compounds showed a similar behavior in lipid model membranes, making them useful in nucleotide-based technologies.
New 3'-3' linkers for alternate strand triplex forming oligonucleotides
De Napoli,Di Fabio,Messere,Montesarchio,Musumeci,Piccialli
, p. 1639 - 1640 (2007/10/03)
New solid supports, functionalized with suitably protected 1,2,3- propanetriol and cis, cis-1,3,5-cyclohexanetriol, were efficiently prepared and used in the standard automated synthesis of 3'-3' linked ODNs for triplex formation experiments.
Transformation of Rings Containing Tetraisopropyldisiloxane-1,3-diyl Group - an Equilibrium Process Enlarging the Scope of Applications of the Group
Markiewicz, Wojciech T.,Bartoszuk, Anna
, p. 453 - 461 (2007/10/02)
The problem of transformation of ring systems containing tetraisopropyldisiloxane-1,3-diyl (TIPDSi) group under acidic, anhydrous conditions has been studied and its mechanism is proposed. This process has been found to be a thermodynamically controlled e
