8-(2-phenylethyl)-1-oxa-3,8-diazaspiro[4.5]decan-2-one CAS NO.5053-06-5

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neural mechanisms contribute to control of mucus secretion in the airways. fenspiride is a non-steroidal antiinflammatory agent which has a variety of actions, including inhibition of neurogenic bronchoconstriction. the effect of fenspiride on neurally-mediated mucus secretion was investigated in vitro in electrically-stimulated ferret trachea, using(35)so(4)as a mucus marker. cholinergic secretory responses were isolated using adrenoceptor and tachykinin receptor antagonists. tachykinin responses were isolated using cholinoceptor and adrenoceptor antagonists. electrical stimulation increased cholinergic secretion by;90% and tachykininergic secretion by;40%. fenspiride (1 microm-1 mm) tended to inhibit cholinergic secretion in a concentration-dependent manner, although only at 1 mm was inhibition (by 87%) significant. inhibition by fenspiride of tachykininergic secretion was not concentration-dependent, and again significant inhibition (by 85%) was only at 1 mm. inhibition was not due to loss of tissue viability, as assessed by restitution of secretory response after washout. fenspiride also inhibited secretion induced by acetylcholine, but did not inhibit substance p-induced secretion. histamine receptor antagonists increased basal secretion by 164%, whereas fenspiride did not affect basal secretion. we conclude that, in ferret trachea in vitro, fenspiride inhibits neurally-mediated mucus secretion, with antimuscarinic action the most plausible mechanism of action, but not necessarily the only mechanism.