- Detection of divinyl ether synthase in Lily-of-the-Valley (Convallaria majalis) roots
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Incubations of linoleic acid with cell-free preparations from Lily-of-the-Valley (Convallaria majalis L., Ruscaceae) roots revealed the presence of 13-lipoxygenase and divinyl ether synthase (DES) activities. Exogenous linoleic acid was metabolized predominantly into (9Z,11E,1′E)-12-(1′-hexenyloxy)-9,11-dodecadienoic (etheroleic) acid. Its identification was confirmed by the data of ultraviolet spectroscopy, mass spectra, 1H NMR, COSY, catalytic hydrogenation. The isomeric divinyl ether (8E,1′E,3′Z)-12-(1′,3′-nonadienyloxy)-8-nonenoic (colneleic) acid was detected as a minor product. Incubations with linoleic acid hydroperoxides revealed that 13-hydroperoxide was a preferential substrate, while the 9-hydroperoxide was utilized with lesser efficiency.
- Ogorodnikova, Anna V.,Latypova, Larisa R.,Mukhitova, Fahima K.,Mukhtarova, Lucia S.,Grechkin, Alexander N.
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experimental part
p. 2793 - 2798
(2009/04/11)
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- Stereospecific synthesis of novel divinyl ether oxylipins by the enzyme from garlic bulbs
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Oxidation of (9Z,11E,13R,S)-13-hydroperoxy-9,11-octadecadienoic acid by the synthase of divinyl ethers from garlic bulbs (Allium sativum L.) was studied. The enzyme was found to be partially stereospecific, preferentially using the (13S)-epimer of hydroperoxide.
- Grechkin,Hamberg
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p. 827 - 828
(2007/10/03)
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