- Site-Selective Synthesis of 15N- and 13C-Enriched Flavin Mononucleotide Coenzyme Isotopologues
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Flavin mononucleotide (FMN) is a coenzyme for numerous proteins involved in key cellular and physiological processes. Isotopically labeled flavin is a powerful tool for studying the structure and mechanism of flavoenzyme-catalyzed reactions by a variety of techniques, including NMR, IR, Raman, and mass spectrometry. In this report, we describe the preparation of labeled FMN isotopologues enriched with 15N and 13C isotopes at various sites in the pyrazine and pyrimidine rings of the isoalloxazine core of the cofactor from readily available precursors by a five-step chemo-enzymatic synthesis.
- Neti, Syam Sundar,Poulter, C. Dale
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p. 5087 - 5092
(2016/07/06)
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- FORMATION OF ALLOXAZINES AND ISOALLOXAZINES
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During investigation of the condensation of aromatic o-amino azo compounds with barbituric acid it was shown that the equilibrium of the cis-trans isomerization process in the initial azo compounds in the solvents used as reaction media during the synthesis of alloxazines and isoalloxazines is shifted toward the trans form.The kinetic investigation of the cis-trans photoisomerization of aromatic o-amino azo compounds was realized by UV spectroscopy and flash photolysis in media with various polarities and proton-donating activities.
- Tul'chinskaya, L. S.,Kozhokina, L. F.,Pirogov, N. O.,Malkin, Ya. N.
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p. 381 - 384
(2007/10/02)
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