- Improved scalable synthesis and biological activity of “indoxyl-gal,” a chromogenic histochemical used for the identification of lac+ organisms
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An improved and operationally simple synthesis of 3-indoxyl-β-D-galactopyranoside (in-doxyl-gal) is disclosed. Indoxyl-gal is a sensitive chromogenic histochemical used for the identification of β-galactosidase enzymatic activity (lacZ gene). Synthesis of indoxyl-gal is improved to 25% overall yield in 5 linear steps from affordable starting materials with minimal chromatography. Biological testing confirmed the detection of β-galactosidase activity in all (100%, 19 strains) coliform bacteria using membrane filtration and modified MI agar.
- Banasik, Brent,Mai, Tam,Samadpour, Mansour
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supporting information
p. 822 - 825
(2018/11/06)
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- Oxidative coupling of indoles with 3-oxindoles
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A mild procedure for the union of 3-oxindoles with indoles is reported using oxidative coupling. : The Japan Institute of Heterocyclic Chemistry.
- Jessing, Mikkel,Baran, Phil S.
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experimental part
p. 1739 - 1745
(2011/05/05)
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- 3-substituted indole antiproliferative angiogenesis inhibitors
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3-Substituted indole carbohydrazides having the formula are useful for inhibiting angiogenesis and cell proliferation. Also disclosed are compositions which inhibit angiogenesis and cell proliferation and methods of inhibiting angiogenesis and cancer in a mammal.
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- APPLICATION OF MAGNESIUM ALKOXIDES TO SYNTHESES OF BENZOHETEROCYCLIC COMPOUNDS
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The Dieckmann condensations of methyl -X-acetates Ia-Ih (X = O, S, SO2, NH, NCH3) initiated by action of magnesium methoxide, ethoxide, isopropoxide and other basic reagents have been studied under various conditions.Whereas magnesium methoxide has comparable efficiency as sodium methoxide and potassium tert-butoxide in syntheses of benzoheterocyclic compounds IIa-IIh, magnesium ethoxide gives the ethyl ester IIb in a medium yield, and magnesium isopropoxide is quite inefficient in the condensation reaction.The alkylation of the esters IIa, IId, and IIg with methyl chloroaetate in the presence of sodium hydride in dimethylformamide gives the diesters IIi-IIk which on action by potassium tert-butoxide undergo the cyclization reaction to give esters III.
- Svoboda, Jiri,Nic, Miloslav,Palecek, Jaroslav
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p. 592 - 599
(2007/10/02)
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