10.1002/cbdv.202000483
Chemistry & Biodiversity
Chem. Biodiversity
quinones using the same methodology as described above. The dose of synthetic compounds used was based on the amount of each compound in 15 μL
of the volume solutions (one female tergal gland in 100 μL), since this volume had the best score in initiate male copulatory responses.
Statistical analysis
Statistical analyses were performed with the software BioEstat 5.3. The Chi-square was used to test significant differences between all bioassays.
Differences in mean quantities between males and females of the identified compounds in ten extracts were tested by Shapiro-Wilk normality test,
followed by T-test or Mann-Whitney (Wilcoxon rank-sum test). An experiment-wise error response of 0.05 was considered for all statistical analyses.
Supplementary Material
Acknowledgements
The authors gratefully acknowledge financial support by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brazil), Coordenação de
Aperfeiçoamento de Pessoal de Nível superior (CAPES, Brazil); Instituto Nacional de Ciências e Tecnologia de Semioquímicos na Agricultura (INCT, Brazil)
and Ana Caroline de Lima for the schematic draw in the figure 1.
Author Contribution Statement
M. R. S., D. M. V. and L. F. performed the experiments, data analysis and interpretation, and wrote the manuscript; P. T. B. contributed to the writing,
review and editing; J. B. aided to the analyses of results and to writing of the manuscript; P. H. G. Z. designed and supervised the research.
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