[Bis(2-hydroxyethyl)ammonio]acetate

Base Information

• Chemical Name: [Bis(2-hydroxyethyl)ammonio]acetate
• CAS No.: 150-25-4
• Molecular Formula: C6H13NO4
• Molecular Weight: 163.174
• Hs Code.: 29225000
• Mol file: 150-25-4.mol

Synonyms:[bis(2-hydroxyethyl)ammonio]acetate;2-[bis(2-hydroxyethyl)azaniumyl]acetate;bicine buffer;CHEBI:39066

Chemical Property of [Bis(2-hydroxyethyl)ammonio]acetate

Chemical Property:

• Appearance/Colour: White crystalline powder
• Vapor Pressure: 0.002-0.007Pa at 20-25℃
• Melting Point: 190 °C (dec.)(lit.)
• Refractive Index: 1.4240 (estimate)
• Boiling Point: 388.5 °C at 760 mmHg
• PKA: 8.3(at 25℃)
• Flash Point: 188.8 °C
• PSA: 81.00000
• Density: 1.31 g/cm³
• LogP: -1.64240
• Storage Temp.: 2-8°C
• Solubility.: H2O: 1 M at 20 °C, clear, colorless
• Water Solubility.: soluble
• XLogP3: -3.1
• Hydrogen Bond Donor Count: 3
• Hydrogen Bond Acceptor Count: 4
• Rotatable Bond Count: 5
• Exact Mass: 163.08445790
• Heavy Atom Count: 11
• Complexity: 106

Purity/Quality:

99% ^*data from raw suppliers

Bicine ≥99% ^*data from reagent suppliers

Safty Information:

• Pictogram(s): Xn
• Hazard Codes: Xn
• Statements: 20/21/22-36/37/38
• Safety Statements: 24/25-36-26

MSDS Files:

SDS file from LookChem

Useful:

• Canonical SMILES: C(CO)[NH+](CCO)CC(=O)[O-]
• Uses: To prepare stable substrate solution for the determination of guanine deaminase. The buffer is widely used with the working concentration of 3-100mM. Biological buffer and chelating agent. A zwitterionic amino acid buffer agent used for low temperature work. The use of bicine in a thin layer ion exchange chromatography method for protein resolution has been published. Bicine has been utilized in peptide and protein crystallization. A kinetic study of a quaternary transition-state analogue complex of creatine kinase used bicine in the reaction buffer. A multiphasic buffer system for SDS-PAGE of proteins and peptides that incorporates bicine has been described.

Technology Process of [Bis(2-hydroxyethyl)ammonio]acetate

There total 13 articles about [Bis(2-hydroxyethyl)ammonio]acetate which guide to synthetic route it. The literature collected by LookChem mainly comes from the sharing of users and the free literature resources found by Internet computing technology. We keep the original model of the professional version of literature to make it easier and faster for users to retrieve and use. At the same time, we analyze and calculate the most feasible synthesis route with the highest yield for your reference as below:

synthetic route:

Reference yield: 96.0%

Glyoxal (131543-46-9,107-22-2) + 2,2'-iminobis[ethanol] (111-42-2) → N,N-bis-(2-hydroxyethyl)glycine (150-25-4)

Guidance literature:

In ethanol; water; at 70 ℃; for 16h;

DOI:10.1055/s-1987-28126

Reference yield:

bromoacetic acid methyl ester (96-32-2) + 2,2'-iminobis[ethanol] (111-42-2) → N,N-bis-(2-hydroxyethyl)glycine (150-25-4)

Guidance literature:

With sodium hydroxide; triethylamine; In propan-1-ol; ethanol;

Reference yield:

4-(2-hydroxy-ethyl)-morpholin-2-one (5038-12-0) → N,N-bis-(2-hydroxyethyl)glycine (150-25-4)

Guidance literature:

With water; at 25 ℃; Rate constant; at pD 7.0; hydrolysis also in humid air;

DOI:10.1016/S0040-4039(97)00330-4

upstream raw materials:

oxirane

GlyOEt*HCl

4-(2-hydroxy-ethyl)-morpholin-2-one

N,N-bis-(2-chloro-ethyl)-glycine

Downstream raw materials:

1-phenyl-2,8,9-trioxa-5-aza-1-germatricyclo[3.3.3.0(1,5)]undecane-3-one

4-(2-hydroxy-ethyl)-morpholin-2-one

1-(3-Fluoro-phenyl)-2,8,9-trioxa-5-aza-1-sila-bicyclo[3.3.3]undecan-3-one

m-trifluoromethylphenylsilatranone

Refernces

• 1、 -. "Stabilizing labeled antibody using amino acids". . . Retrieved 2011/11/07.
• 2、 -. "Isolation of nucleic acids". . . Retrieved 2008/06/13.