Detail of "10047-33-3"

Reference

Biological activity of progastrin posttranslational processing intermediates

Biological activity of progastrin posttranslational processing intermediates. Matsumoto, Masahiro; Park, Jung; Sugano, Kentaro; Yamada, Tadataka (Med. Cent., Univ. Michigan, Ann Arbor, MI 48109, USA). Am. J. Physiol., 252(3, Pt. 1), G315-G319 (English) 1987. CODEN: AJPHAP.Several reagents with their cas registry numbers 53988-98-0 and 108093-87-4 are used here. ISSN: 0002-9513. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Carboxyl-terminally extended progastrin [53988-98-0] posttranslational processing intermediates in G cells of the gastric antrum have been identified by S. et al (1985) and by S. and Y. (1985), and it was demonstrated that they are cosecreted with gastrin. To det. the physiol. significance of these intermediates, the biol. activities for 2 synthetic gastrin precursor analogs that correspond to hexagastrin with carboxyl-terminal extensions, Tyr-Gly-Trp-Met-Asp-Phe-Gly [108093-87-4] and Tyr-Gly-Trp-Met-Asp-Phe-Gly-Arg-Arg [95574-93-9], were detd. on gastric parietal and D cells isolated from canine fundic mucosa. Both analogs were as efficacious as human gastrin heptadecapeptide [10047-33-3] in displacing 125I-labeled [Leu15]gastrin from binding sites on the 2 cell types and in stimulating [14C]aminopyrine uptake by parietal cells and somatostatin release from D cells. However, both analogs were 104-105-fold less potent than gastrin heptadecapeptide in these activities. Apparently, progastrin processing intermediates do not have physiol. relevant actions under normal circumstances, and carboxyl-terminally amidated peptides such as gastrin [9002-76-0] probably require the amide moiety for biol. activity. .

Intragastric titration of acid in the perfused rat stomach preparation following parenteral and intraluminal stimulation

Intragastric titration of acid in the perfused rat stomach preparation following parenteral and intraluminal stimulation. Halter, Fred; Galeazzi, Renato; Leuenberger, Johanna; Smith, Gordon M. (Gastrointest. Unit, Univ. Hosp., Bern, Switz.). Eur. J. Pharmacol., 43(3), 247-52 (English) 1977. CODEN: EJPHAZ. DOCUMENT TYPE: Journal CA Section: 2 (Hormone Pharmacology) Section cross-reference(s): 13 The anesthetized rat stomach prepn. was adapted to assay intraluminally administered stimulants and inhibitors of acid secretion by continuously recirculating the perfusate (15 mL of 5% glucose) and measuring acid by the pH-stat technique. The responses obtained were linear. Basal acid secretion declined following stepwise lowering of the intragastric pH between 6.5 and 4.0. The threshold values for responses to i.v. human gastrin I (I) [10047-33-3] were <16 ng. At least 6 doses were given per rat without tachyphylaxis. Acetylcholine [51-84-3] (1.3, 2.5, 5.2, and 10.4 .mu.g/mL) in the perfusate induced a dose-dependent linear acid secretion. Metiamide [34839-70-8] (1.66 mg/mL) in the perfusate produced a parallel shift to the right of the I dose-response curve producing an agonist dose ratio of 3.66. Appqrently, intragastrically applied stimulants and inhibitors of acid secretion can be studied precisely in this relatively simple prepn.