Reference

The availability of therapeutic hydroxocobalamin to cells

The availability of therapeutic hydroxocobalamin to cells. Hall, Charles A.; Begley, James A.; Green-Colligan, Pamela D. (Nutrition Lab. Clin. Assess. Res., Veterans Adm. Med. Cent., Albany, NY 12208, USA). Blood, 63(2), 335-41 (English) 1984. CODEN: BLOOAW. ISSN: 0006-4971. DOCUMENT TYPE: Journal CA Section: 18 (Animal Nutrition) Hydroxocobalamin (OH-Cbl) [13422-51-0] when used to treat human vitamin B12 deficiency, is better retained by the body than is cyanocobalamin (CN-Cbl) [68-19-9]. The uptake and internalization of OH-Cbl and CN-Cbl bound to transcobalamin II (TCll) [12651-28-4] by HeLa cells was compared. TCll-OH-Cbl was taken up in larger amts. per unit time; the greater uptake was not a consequence of more effective attachment to receptors of TCll-Cbl nor to a more rapid regeneration of receptors. The difference was expressed during the phase of internalization of TCll-Cbl. With CN-Cbl, the stages of binding to receptors plus internalization were more readily reversed. Larger amts. of OH-Cbl were internalized and converted to active coenzyme forms of Cbl. When added in vitro in equiv. amts., more OH-Cbl was bound to nonspecific human plasma proteins. This greater and broader binding neither enhanced nor interfered with the uptake of Cbl by cells, which was detd. by the amt. 12651-28-4 and 68-19-9 are just another two chemicals used in this study. of Cbl bound physiol. to TCll. OH-Cbl is thus a more efficient form of treatment of the common types of Cbl deficiency, principally because of the better retention, which requires less frequent injections, but also because of greater availability to cells. .

Mechanism of sodium nitroprusside induced neuromuscular block and its influence on skeletal muscle relaxants

Mechanism of sodium nitroprusside induced neuromuscular block and its influence on skeletal muscle relaxants. Diwan, Prakash V.; Sharma, Pyare L.; Varma, Yoginder S. (Dep. Pharmacol., Postgrad. Inst. Med. Educ. Res., Chandigarh, India). Indian J. Med. Res., 78(Oct.), 581-6 (English) 1983. CODEN: IJMRAQ. ISSN: 0019-5340. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) In vivo and in vitro expts. in rabbits and rats, Na nitroprusside (SNP) [14402-89-2] per se produced a dose-dependent neuromuscular block without affecting the directly elicited twitch response. The tetanus was well sustained and there was no post-tetanic facilitation. 57-94-3 and 16478-59-4 which are cas registry numbers of substances are two of reagents here. In the presence of physostigmine, a partial transient recovery was followed by worsening of the SNP block. SNP did not affect neuromuscular block induced by succinylcholine [306-40-1], but it potentiated the effects of pancuronium [15500-66-0] in both in vivo and in vitro studies. Na thiosulfate [7772-98-7] and hydroxycobalamine [13422-51-0] partially antagonized the NaCN and SNP-induced neuromuscular block in vitro, but hydroxycobalamine failed to modify the SNP action in in vivo rabbit studies. The mechanism involved in SNP induced neuromuscular block and its interaction with pancuronium and succinylcholine are discussed. .