Detail of "1510-21-0"

Reference

Specific lipid requirements in reconstitution of the b-adrenergic receptor function

Specific lipid requirements in reconstitution of the b-adrenergic receptor function. Schramm, M.; Kirilovsky, J.; Citri, Y. (Dep. Biol. Chem., Heb. Univ. Jerusalem, Jerusalem 91904, Israel). Neurol. Neurobiol., 8A(Catecholamines, Pt. A), 279-82 (English) 1984. CODEN: NEUND9. ISSN: 0736-4563. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) The b-adrenergic receptor (R) function requiring R, the hormone-stimulated GTPase [9059-32-9] was reconstituted from sep. crude R and guanyl nucleotide-binding protein prepns. by treatment with sufficient phospholipids. Delipidated R was successfully relipidated and activated (adenylate cyclase activation) by the addn. of a variety of lipids. These included: phosphatidylethanolamine which was as efficient as a mixt. of cholesterol hemisuccinate [1510-21-0] and cardiolipin, a mixt. of monooleoyl glycerol [25496-72-4] and cholesterol hemisuccinate, and phosphatidylcholines. The individual acidic phospholipids, phosphatidic acids, cardiolipins, and phosphatidylinositols were inactive.

Functional properties of the acetylcholine receptor incorporated in model lipid membranes

Functional properties of the acetylcholine receptor incorporated in model lipid membranes. Differential effects of chain length and head group of phospholipids on receptor affinity states and receptor-mediated ion translocation. Criado, Manuel; Eibl, Hansjoerg; Barrantes, Francisco J. (Max-Planck-Inst. Biophys. Chem., Goettingen-Nikolausberg 3400, Fed. Rep. Ger.). J. Biol. Chem., 259(14), 9188-98 (English) 1984. CODEN: JBCHA3. ISSN: 0021-9258. DOCUMENT TYPE: Journal CA Section: 2 (Mammalian Hormones) Section cross-reference(s): 6 Torpedo Acetylcholine receptor was reconstituted into liposomes of pure synthetic lipids to study the influence of the lipid environment on affinity state transitions and the ion translocation function of the receptor. A crit. concn. 40290-42-4 is the cas registry number of certain chemical which is used as reagents here. of 30-40% of cholesteryl hemisuccinate [1510-21-0] was necessary in liposomes made of cholesteryl hemisuccinate and dimyristoyl phosphatidylcholine [18194-24-6] to mimic the kinetics of agonist-induced state transitions obsd. in native membranes. With increasing chain length of the satd. lecithins, a marked increase in carbamylcholine [462-58-8] dissocn. consts. was obsd. Substitution by other dimyristoyl phospholipids for dimyristoyl phosphatidylcholine had the same, though quant. less pronounced effects. Introduction of unsatn. in the acyl chains reverted the effect of increasing chain length. Unsatd. phosphatidylethanolamines in combination with 28-35 mol % of cholesteryl hemisuccinate was the best lipid mixt. for reconstitution of the receptor-gating function. When phosphatidylethanolamine was replaced totally or partially by other phospholipids with the same or different acyl chain compn., a marked decrease of ion transport was apparent, even when similar vesicle size, receptor incorporation, and agonist-induced affinity transitions were obtained. Therefore, the maintenance of the affinity state transitions of the reconstituted receptor is a necessary but not sufficient condition for the manifestation of the ion-gating receptor activity. On the other hand, the more unsatd. the acyl chains of phosphatidylethanolamine are, the higher the response that was obsd., suggesting that a crit. lipid packing is essential for the ion translocation function of the receptor. .