Reference

Auramine O as a fluorescent probe for the binding of basic drugs to human a1-acid glycoprotein (a1-AG)

Auramine O as a fluorescent probe for the binding of basic drugs to human a1-acid glycoprotein (a1-AG). The development of a simple fluorometric method for the determination of a1-AG in human serum. Sugiyama, Yuichi; Suzuki, Yasuyuki; Sawada, Yasufumi; Kawasaki, Seiji; Beppu, Tomoe; Iga, Tatsuji; Hanano, Manabu (Fac. Pharm. Sci., Univ. Tokyo, Tokyo 113, Japan). Biochem. Pharmacol., 34(6), 821-9 (English) 1985. CODEN: BCPCA6. ISSN: 0006-2952. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Section cross-reference(s): 9 A cationic fluorescent dye, auramine O (AO) [2465-27-2], exhibited an intense increase in fluorescence after binding to human a1-acid glycoprotein (a1-AG). The interaction between AO and the protein was studied by fluorescence spectroscopy and by equil. dialysis. AO bound to the protein via a single site with a dissocn. const. of 24 mM. Chlorpromazine [50-53-3], imipramine [50-49-7], desipramine [50-47-5], quinidine [56-54-2], propranolol [525-66-6], and lidocaine [137-58-6], which are known to bind to the protein, competitively inhibited the AO binding to the protein. The dissocn. consts. of these basic drugs obtained from such inhibitory expts. were comparable to those obtained with other methods (equil. dialysis, quenching of protein intrinsic fluorescence, and the difference spectrophotometric method) and from the literature. AO may be a useful fluorescent probe that binds to a single basic drug binding site on a1-AG. In addn., a simple fluorometric method for the detn. of a1-AG in serum was developed using AO, and the validity of this method was confirmed by comparing it with the conventional radial immunodiffusion method.

Influence of surfactants on the absorption and fluorescence spectra of Auramine-O dye

Influence of surfactants on the absorption and fluorescence spectra of Auramine-O dye. Vyas, Seema; Kapoor, R. C. (Dep. Chem., Univ. Jodhpur, Jodhpur, India). Polym. Photochem., 4(6), 473-81 (English) 1984. CODEN: POPHDO. ISSN: 0144-2880. DOCUMENT TYPE: Journal CA Section: 41 (Dyes, Organic Pigments, Fluorescent Brighteners, and Photographic Sensitizers) Section cross-reference(s): 46, 66 A study of the effect of some nonionic, cationic and anionic surfactants on the fluorescence and absorption behavior of Auramine O [2465-27-2] showed that the anionic surfactants exert max. influence on the fluorescence and absorption spectra of the dye. The effects of various surfactants were explained on the hypothesis of dye-surfactant interaction and a subsequent disaggregation process. A red shift in fluorescence and absorption max. indicated the possibility of dye-surfactant interaction although the nature of the spectra remained unaltered.