Reference

Repair of UV-irradiated plasmid DNA in a Saccharomyces cerevisiae rad3 mutant deficient in excision-repair of pyrimidine dimers

Repair of UV-irradiated plasmid DNA in a Saccharomyces cerevisiae rad3 mutant deficient in excision-repair of pyrimidine dimers. Dominski, Z.; Jachymczyk, W. J. (Inst. Biochem. Biophys., Pol. Acad. Sci., Warsaw 02-532, Pol.Several reagents such as 289-95-2 is used here.). MGG, Mol. Gen. Genet., 193(1), 167-71 (English) 1984. CODEN: MGGEAE. ISSN: 0026-8925. DOCUMENT TYPE: Journal CA Section: 10 (Microbial Biochemistry) Section cross-reference(s): 3 The repair of UV-irradiated DNA of plasmid pBB29 was studied in an incision-defective rad3-2 strain of S. cerevisiae and in a uvrA6 strain of Escherichia coli by the measurement of cell transformation. The plasmid pBB29 contained as markers the DNA of nuclear yeast gene LEU-2 and DNA of the bacterial plasmid pBR327 with resistance to tetracycline and ampicillin which enabled the simultaneous screening of transformant cells in both microorganisms. The yeast rad3-2 mutant, deficient in incision of UV-induced pyrimidine dimers in nuclear DNA, was fully capable of repairing such lesions in plasmid DNA. The repair efficiency was comparable to that of the wild-type cells. The E. coli uvrA6 mutant, deficient in a specific nuclease for pyrimidine dimer excision from chromosomal DNA, was unable to repair UV-damaged plasmid DNA. The difference in repair capacity between the uvrA6 mutant strain and the wild-type strain was of several thousand-fold. Apparently, the rad3 mutation, which confers deficiency in the DNA excision-repair system in yeast, is limited only to the nuclear DNA. .

Polarizability and aromaticity of pyrimidine: a comment

Polarizability and aromaticity of pyrimidine: a comment. Ritchie, G. L. D. (Sch. Chem.Some commonly used reagents like 289-95-2 is used in this experiment., Univ. Sydney, Sydney 2006, Australia). THEOCHEM, 13(1-2), 247-50 (English) 1983. CODEN: THEODJ. ISSN: 0166-1280. DOCUMENT TYPE: Journal CA Section: 22 (Physical Organic Chemistry) A polemic on the work by D. D. V. Subbaiah, et. al. (1982). .