Reference

Use of derivative resolution for the spectrophotometric determination of drug mixtures

Use of derivative resolution for the spectrophotometric determination of drug mixtures. III. Second-derivative determination of binary mixtures of estrogens and progestins. Corti, P.; Lencioni, E.; Sciarra, G. F. (Ist. Chim. Farm. Tossicol., Univ. Siena, Siena, Italy). Boll. Chim. Farm., 122(6), 281-8 (Italian) 1983. CODEN: BCFAAI. ISSN: 0006-6648. DOCUMENT TYPE: Journal CA Section: 64 (Pharmaceutical Analysis) Section cross-reference(s): 2 Mestranol [72-33-3] or ethynylestradiol [57-63-6] mixts. with quingestanol [10592-65-1], quingestanol acetate [3000-39-3], methylestrenolone [514-61-4], or norethindrone [68-22-4] can be sepd. and the components identified by chromatog. on silica gel plates with cyclohexane-CHCl3-MeOH-NH4OH (5:2:1:0.5), spraying with 1% H2SO4 in MeOH and heating at 50° for 10 min. The limit of detection of the colored spots was 0.3-0.6 mg. Binary estrogen/progestin mixts. (1:10-1:50) are quantitated by 2nd deriv. spectrophotometry of solns. in MeOH-CHCl3. Spectral and deriv. data are tabulated. Relative std deviations of mixts. of 6.2-25 mg/mL mestranol with 10-50 parts progestin were 1.91-4.18%, and those for 5.6-22.6 mg/mL ethynylestradiol with the same ratio of progestins were 0.83-4.97%.Except for chemicals metioned above, 10592-65-1 and 57-63-6 are also used. .

Short-term and prolonged treatment with oral contraceptives and liver function

Short-term and prolonged treatment with oral contraceptives and liver function. Kulcsar, A.; Kulcsar-Gergely, J. (II Med. Clin., Univ. Med. Sch., Debrecen, Hung.). Arzneim.-Forsch., 27(9), 1694-7 (English) 1977. CODEN: ARZNAD. DOCUMENT TYPE: Journal CA Section: 2 (Hormone Pharmacology) Expts. on female rats were performed to observe the effects of progesterone (I) [57-83-0] and two synthetic progestogens: ethinodiol diacetate [297-76-7] and methylestrenolone [514-61-4] on liver function. In acute expts. animals were treated for the duration of one sexual cycle. All 3 compds. prolonged the hexobarbital biotransformation time. This effect was reversible and normalized 48 h after stopping treatment. Testosterone inducement of microsomal mixed function oxidases remained at the level of controls after methylestrenolone pretreatments and was even higher in the ethinodiol diacetate and I pretreated animals. Subacute and chronic treatments lasting for 1 or more sexual cycles resulted in the same slight inhibition of hexobatbital biotransformation when performed with the progestogens. Chronic I administration prolonged hexobarbital biotransformation time. This impairment was also reversible. Apparently, there is a moderate, transient inhibition of hexobarbital metab. during short-term and prolonged I and progestogen treatment. This effect may be easily suspended by interrupting drug administration or inducing microsomal enzymes with a single dose of testosterone. In I expts., the inhibition of liver function increased with dose and time of exposure.