Detail of "6160-78-7"

Reference

Coupling of enzymatic and immunoassay steps to detect E

Coupling of enzymatic and immunoassay steps to detect E. coli: a new, highly sensitive tandem technique for the analysis of low levels of bacteria. Abuknesha, Ramadan A.; Darwish, Fatima (Analytical Research Group, Department of Pharmacy, King's College London, London SE19NN, UK). Talanta, 65(2), 343-348 (English) 2005 Elsevier B.V. CODEN: TLNTA2. ISSN: 0039-9140. DOCUMENT TYPE: Journal CA Section: 9 (Biochemical Methods) Section cross-reference(s): 11 A tandem technique for the detection of very low levels E. coli within about 2 h is demonstrated. The technique couples the widely employed microbial enzymic detection methods with an immunoassay step. The bacterial marker enzyme, E. coli b-D-galactosidase, was used in conjunction with synthetic enzyme substrates to produce products that could be measured with a highly sensitive enzyme-labeled immunosorbent assay (ELISA). The com. available 4-methylumbelliferyl-b-D-galactoside and a newly prepd. 3068-32-4 and 6160-78-7 which are cas registry numbers of chemicals are mentioned. substrate, 4-methylcoumarin-3-propionate-7-O-b-D-galactoside, were used with an ELISA for 7-hydroxy-4-methylcoumarin to demonstrate the detection of low levels of E. coli. The 2 h test indicates that a few viable bacteria cells could be detected by the tandem procedure. The end point of the test is an ELISA with colorimetric measurement step. The novel approach retains the essential features of the microbial enzymic detection procedures and provides a highly sensitive detection system that can be used for rapid screening or quantification of viable microbial cells in water samples. The tandem test is generic for commonly employed glycosidases and other marker enzymes for which 4-methylumbillerone substrates are available. .

Quantification of minute levels of b galactosidase in individual 2- to 16-cell-stage mouse embryos

Quantification of minute levels of b galactosidase in individual 2- to 16-cell-stage mouse embryos. Forlani, Sylvie; Nicolas, Jean-Francois (Unite Biol. Molecularie Development, Inst. Pasteur, Paris 75015, Fr.Some commonly used reagents like 9031-11-2 and 6160-78-7 are used in this experiment.). Trends in Genetics, 12(12), 498-500 (English) 1996 Elsevier. CODEN: TRGEE2. ISSN: 0168-9525. DOCUMENT TYPE: Journal CA Section: 7 (Enzymes) Section cross-reference(s): 13 An assay for b-galactosidase activity using the fluorogenic substrate 4-methylumbelliferyl b-D-galactoside (MUG) that has been previously applied to large pools of mouse transgenic embryos was modified to increase its sensitivity and reproducibility, making it ideal for the quantification of minute levels of b-galactosidase. Anal. of lacZ expression in individual mouse pre-implantation embryos was performed to illustrate the method. .