Reference

Basophil interleukin 4 and interleukin 13 production is suppressed during the early phase of rush immunotherapy

All Rights Reserved. Basophil interleukin 4 and interleukin 13 production is suppressed during the early phase of rush immunotherapy. Plewako, Halina; Wosinska, Katarzyna; Arvidsson, Monica; Bjoerkander, Janne; Stahl Skov, Per; Hakansson, Lena; Rak, Sabina ( Asthma and Allergy Research Group, Department of Respiratory Medicine and Allergology, Sahlgrenska University Hospital, Goeteborg SE-413 45, Swed.). International Archives of Allergy and Immunology, 141(4), 346-353 (English) 2006 S. Karger AG. CODEN: IAAIEG. ISSN: 1018-2438. DOCUMENT TYPE: Journal CA Section: 1 (Pharmacology) Section cross-reference(s): 15 Background: Studies using rush immunotherapy (RIT) have shown that rapid protection can be achieved using protocols allowing a fast increment of allergen dose. We examd.Some commonly used reagents like 1247-42-3 and 87848-99-5 are used in this experiment. the early effects of RIT on basophil nos. and expression of CD203c, prodn. of interleukin (IL)-4 and IL-13 and histamine release by basophils in the peripheral blood of patients treated with immunotherapy and controls. Methods: Twelve patients treated with RIT and 4 untreated controls were included in the study. Any adverse events were evaluated during the incremental phase of RIT. Mononuclear cells were isolated before the start of RIT and 3 days, 1 wk, 4 wk and 3 mo after the beginning of the treatment. Histamine release upon allergen stimulation, expression of CD203c and allergen-induced prodn. of IL-4 and IL-13 by basophils were examd. Results: Significant decreases in blood basophil count (p = 0.02) were obsd. early in the treatment, returning to baseline values 1 wk after the start of RIT. Similarly, histamine release decreased at day 3 (p = 0.02), but returned to pretreatment levels after 1 wk. Also, the percentage of IL-4+ and IL-13+ basophils and levels of CD203c expression were markedly reduced early in the treatment. IL-4 and IL-13 prodn. correlated with histamine release and CD203c expression. Histamine release and prodn. of IL-4 and IL-13 by basophils before the treatment correlated with the severity of adverse events during the incremental phase of RIT. Conclusion: We report the decrease in blood basophil nos., their lower activation status and the reduced prodn. of IL-4 and IL-13 early in the course of RIT. This early suppression of basophil activation could be one mechanism behind the protective effect of RIT. .