Reference

A carbon-13 NMR study of the biosynthesis of lupinine and sparteine

A carbon-13 NMR study of the biosynthesis of lupinine and sparteine. Golebiewski, W. Marek; Spenser, Ian D. (Dep. Chem., McMaster Univ., Hamilton L8S 4M1, Can.There are some reagents with their cas registry numbers 90-39-1 and 462-94-2 are used in this study.). J. Chem. Soc., Chem. Commun., (24), 1509-11 (English) 1983. CODEN: JCCCAT. ISSN: 0022-4936. DOCUMENT TYPE: Journal CA Section: 11 (Plant Biochemistry) Section cross-reference(s): 31 13C NMR study of lupinine (I) and sparteine (II) formed from labeled cadaverine [H2N(CH2)413CH215NH2] in Lupinus luteus showed that I is generated from 2 and II from 3 cadaverine-derived C5-units, each by a route which excludes intermediates with C2V symmetry. .

A radiometric assay for debrisoquine 4-hydroxylase in human liver microsomes

A radiometric assay for debrisoquine 4-hydroxylase in human liver microsomes. Nakano, M.; Inaba, T. (Fac. Med., Univ. Toronto, Toronto, ON M5S 1A8, Can.). Can. J. Physiol. Pharmacol., 62(1), 84-8 (English) 1984. CODEN: CJPPA3.Several substances with their cas registry numbers 90-39-1 and 1131-64-2 may be metioned in this study. ISSN: 0008-4212. DOCUMENT TYPE: Journal CA Section: 7 (Enzymes) A radiometric method to assay debrisoquine 4-hydroxylase (I) activity in human liver microsomes was established. Following incubation with 14C-labeled debrisoquine, unreacted debrisoquine was extd. with CHCl3; 4-hydroxydebrisoquine was derivatized with hexafluoroacetylacetone, extd., and subjected to high-performance thin-layer chromatog. followed by liq. scintillation counting. The Km values for I were 70 and 120 mM and the Vmax values were 8 and 24 pmol/mg microsomal protein/min. By application of this assay, it was possible to show that 4-hydroxydebrisoquine formation was competitively inhibited by sparteine. Antipyrine up to a concn. of 4 mM had no effect. .