10121-91-2 Usage
Description
DANSYLCADAVERINE, also known as Monodansylcadaverine (MDC), is a fluorescent marker for autophagic vacuoles. It is an autofluorescent substance incorporated into multilamellar bodies by both an ion trapping mechanism and interaction with membrane lipids, exhibiting a Stokes shift and increased relative fluorescence in hydrophobic environments. MDC selectively accumulates in subcellular fractions containing lysosomal enzymes, such as acid phosphatase and cathepsin D, but not those containing endoplasmic reticulum markers.
Uses
Used in Biochemical Assays:
DANSYLCADAVERINE is used as a fluorescent substrate for the assay of transamidating enzymes, allowing for the detection and quantification of enzyme activity.
Used in Lysosomal Research:
DANSYLCADAVERINE is used as a lysosomotropic fluorescent probe, enabling the study of lysosomal function and the effects of various treatments on lysosomal activity.
Used in Autophagy Research:
DANSYLCADAVERINE is used as a fluorescent amine probe to study autophagy in the cytoplasm and vacuoles of plants and mammalian cells. It selectively accumulates in autophagic vacuoles, providing a visual marker for the assessment of autophagy processes.
Used in Cell Biology:
DANSYLCADAVERINE is used as a fluorescent marker to visualize and study the formation and dynamics of autophagic vacuoles in cells, contributing to a better understanding of cellular autophagy mechanisms and their regulation.
References
1) Munafo and Colombo (2001),?A novel assay to study autophagy: regulation of autophagosome vacuole size by amino acid deprivation; Int. J. Cell Sci.,?114?3619
2) Jiang?et al. (2012),?Targeting androgen receptor leads to suppression of prostate cancer via induction of autophagy; J. Urol.,?188?1361
3) Haigler?et al.?(1980),?Dansylcadaverine inhibits internalization of 125I-epidermal growth factor in BALB 3T3 cells; J. Biol. Chem., 255 1239
4) Murthy?et al. (1994),?Residue Gln-30 of human erythrocyte anion transporter is a prime site for reaction with intrinsic transglutaminase; J. Biol. Chem.,?269?22907
Check Digit Verification of cas no
The CAS Registry Mumber 10121-91-2 includes 8 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 5 digits, 1,0,1,2 and 1 respectively; the second part has 2 digits, 9 and 1 respectively.
Calculate Digit Verification of CAS Registry Number 10121-91:
(7*1)+(6*0)+(5*1)+(4*2)+(3*1)+(2*9)+(1*1)=42
42 % 10 = 2
So 10121-91-2 is a valid CAS Registry Number.
InChI:InChI=1/C18H27N3O2S/c1-21(2)17-11-7-10-16-15(17)9-8-12-18(16)24(22,23)20-14-6-4-3-5-13-19/h7-12,20H,3-6,13-14,19H2,1-2H3
10121-91-2Relevant articles and documents
Expanding the rubterolone family: Intrinsic reactivity and directed diversification of PKS-derived pyrans
Guo, Huijuan,Benndorf, René,K?nig, Stefanie,Leichnitz, Daniel,Weigel, Christiane,Peschel, Gundela,Berthel, Patrick,Kaiser, Marcel,Steinbeck, Christoph,Werz, Oliver,Poulsen, Michael,Beemelmanns, Christine
, p. 11319 - 11324 (2018)
We characterized two key biosynthetic intermediates of the intriguing rubterolone family (tropolone alka-loids) that contain a highly reactive pyran moiety (in equilibrium with the hydrolyzed 1,5-dione form) and undergo spontaneous pyridine formation in the presence of primary amines. We exploited the intrinsic reactivity of the pyran moiety and isolated several new rubterolone derivatives, two of which contain a unique thiazolidine moiety. Three rubterolone derivatives were chemically modified with fluorescence and biotin tags using peptide coupling and click reaction. Overall, eight derivatives were fully characterized by HRMS/MS and 1D and 2D NMR spectroscopy and their antimicrobial, cytotoxic, anti-inflammatory and antiparasitic activities evaluated.