101515-08-6 Usage
Description
-N7-DGTP, also known as 7-Deaza-2'-deoxyguanosine 5'-triphosphate, is a nucleotide that serves as a substrate for most DNA polymerases, including Taq DNA polymerase. It is characterized by its ability to enhance polymerase chain reaction (PCR) product yield, particularly in the context of GC-rich DNA sequences.
Uses
Used in DNA Sequencing:
-N7-DGTP is used as a substitute for dGTP in dideoxy-chain termination sequencing methods. The application reason is to overcome compression problems in gel electrophoresis when sequencing GC-rich stretches of DNA, which can lead to difficulties in obtaining accurate and clear results.
Used in Polymerase Chain Reaction (PCR):
-N7-DGTP is used as a partial substitute for dGTP in PCRs to improve the product yield when the template is GC-rich. The application reason is that GC-rich templates tend to form extensive secondary structures, which can hinder the efficiency of PCR amplification. By incorporating -N7-DGTP, the formation of these secondary structures is reduced, leading to more efficient and accurate PCR product generation.
Biochem/physiol Actions
This dGTP analog pairs weakly with conventional bases to minimize DNA secondary structures, while being a good substrate for DNA ploymerases.
Check Digit Verification of cas no
The CAS Registry Mumber 101515-08-6 includes 9 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 6 digits, 1,0,1,5,1 and 5 respectively; the second part has 2 digits, 0 and 8 respectively.
Calculate Digit Verification of CAS Registry Number 101515-08:
(8*1)+(7*0)+(6*1)+(5*5)+(4*1)+(3*5)+(2*0)+(1*8)=66
66 % 10 = 6
So 101515-08-6 is a valid CAS Registry Number.
