151619-81-7Relevant articles and documents
Organic co-solvents restore the inherently high enantiomeric ratio of lipase B from Candida antarctica in hydrolytic resolution by relieving the enantiospecific inhibition of product alcohol
Lundhaug, Kamilla,Overbeeke, P.L. Antoine,Jongejan, Jaap A.,Anthonsen, Thorleif
, p. 2851 - 2856 (1998)
The enantiomeric ratio in the hydrolysis of racemic 3-chloro-1- phenylmethoxy-2-propyl butanoate with lipase B from Candida antarctica, CALB, is raised from E=50 to E=180 upon addition of acetone to the aqueous medium. This co-solvent effect is now explained as an enantiospecific inhibition of the lipase by the liberated alcohol. In the range from 0 to 30% acetone, the effect correlates with the increased solubility of the alcohol in the reaction medium. Free and immobilized CALB show similar behaviour.