27398-39-6

Basic information

• Product Name: 3-CHLORO-2-PYRAZINE-CARBOXYLIC ACID
• Synonyms: RARECHEM AL BO 0855;3-CHLORO-2-PYRAZINE-CARBOXYLIC ACID;3-CHLORO-PYRAZINE-2-CARBOXYLIC ACID;3-Chloro-2-pyrazine-carboxylic a;3-Chloropyrazine-2-corboxylic acid;3-Chloropyrazine-2-carbox...;3-Chloro-2-pryazine-carboxylic acid;2-Pyrazinecarboxylic acid, 3-chloro-
• CAS NO: 27398-39-6
• Molecular Formula: C₅H₃ClN₂O₂
• Molecular Weight: 158.54
• Product Categories: pharmacetical;Carboxylic Acids;Pyrazines, Pyrimidines & Pyridazines;Carboxylic Acids;Pyrazines, Pyrimidines & Pyridazines
• Mol File: 27398-39-6.mol
• Article Data: 5

Chemical Properties

• Melting Point: 116-117 °C (decomp)
• Boiling Point: 317 °C at 760 mmHg
• Flash Point: 145.5 °C
• Appearance: /
• Density: 1.579 g/cm³
• Vapor Pressure: 0.000166mmHg at 25°C
• Refractive Index: 1.598
• Storage Temp.: under inert gas (nitrogen or Argon) at 2-8°C
• PKA: 1.51±0.25(Predicted)
• CAS DataBase Reference: 3-CHLORO-2-PYRAZINE-CARBOXYLIC ACID(CAS DataBase Reference)
• NIST Chemistry Reference: 3-CHLORO-2-PYRAZINE-CARBOXYLIC ACID(27398-39-6)
• EPA Substance Registry System: 3-CHLORO-2-PYRAZINE-CARBOXYLIC ACID(27398-39-6)

Safety Data

• Statements: 36/37/38
• Safety Statements: 26-36/37/39
• Hazardous Substances Data: 27398-39-6(Hazardous Substances Data)

Usage

Uses

3-Chloro-2-pyrazinecarboxylic Acid is used in the synthesis of a novel inhibitor (CX-4945) of protein kinase CK2 in the treatment of cancer. Also used in the synthesis of annulated pyrazoles as inhibitors of HIV-1 reverse transcriptase.

InChI:InChI=1/C5H3ClN2O2/c6-4-3(5(9)10)7-1-2-8-4/h1-2H,(H,9,10)

Upstream product

• 55557-52-3 2-chloro-3-cyanopyrazine 
• 27825-21-4 3-chloropyrazine-2-carboxylic acid methyl ester 
• 20737-42-2 3-oxo-3,4-dihydropyrazine-2-carboxylic acid 
• 20737-42-2 3-hydroxypyrazine-2-carboxylic acid 
• 14508-49-7 2-chloropyrazin 
• 124-38-9 carbon dioxide 

Downstream Products

• 931105-81-6 3-Chloro-N-(2,4-difluorophenyl)pyrazine-2-carboxamide 
• 89283-32-9 (3-chloropyrazin-2-yl)methanol 
• 217-68-5 dibenzo[f,h]quinoxaline 

Relevant articles and documents

3-substituted N-benzylpyrazine-2-carboxamide derivatives: Synthesis, antimycobacterial and antibacterial evaluation

A series of substituted N-benzyl-3-chloropyrazine-2-carboxamides were prepared as positional isomers of 5-chloro and 6-chloro derivatives, prepared previously. During the aminolysis of the acyl chloride, the simultaneous substitution of chlorine with benzylamino moiety gave rise to N-benzyl-3-(benzylamino)pyrazine-2-carboxamides as side products, in some cases. Although not initially planned, the reaction conditions were modified to populate this double substituted series. The final compounds were tested against four mycobacterial strains. N-(2-methylbenzyl) -3-((2-methylbenzyl)amino)pyrazine-2-carboxamide (1a) and N-(3,4-dichlorobenzyl)-3-((3,4-dichlorobenzyl)amino)pyrazine-2-carboxamide (9a) proved to be the most effective against Mycobacterium tuberculosis H37Rv, with MIC = 12.5 μg·mL-1. Compounds were screened for antibacterial activity. The most active compound was 3-chloro-N-(2-chlorobenzyl) pyrazine-2-carboxamide (5) against Staphylococcus aureus with MIC = 7.81 μM, and Staphylococcus epidermidis with MIC = 15.62 μ·M. HepG2 in vitro cytotoxicity was evaluated for the most active compounds; however, no significant toxicity was detected. Compound 9a was docked to several conformations of the enoyl-ACP-reductase of Mycobacterium tuberculosis. In some cases, it was capable of H-bond interactions, typical for most of the known inhibitors.

Derivatives of pyrazinecarboxylic acid: 1H, 13C and 15N NMR spectroscopic investigations

NMR spectroscopic studies are undertaken with derivatives of 2-pyrazinecarboxylic acid. Complete and unambiguous assignment of chemical shifts (1H, 13C, 15N) and coupling constants (1H,1H; 13C,1H; 15N,1H) is achieved by combined application of various 1D and 2D NMR spectroscopic techniques. Unequivocal mapping of13C,1H spin coupling constants is accomplished by 2D (S,J) long-range INEPT spectra with selective excitation. Phenomena such as the tautomerism of 3-hydroxy-2-pyrazinecarboxylic acid are discussed.

SYNTHESIS OF 9H-PYRROLO-1,4-DIAZAINDOL-9-ONE

The synthesis of 9H-pyrrolo-1,4-diazaindol-9-one was achieved either by an intramolecular electrophilic substitution of 2-chlorocarbonyl-3-N-pyrrolopyrazine 7 and 2-N-pyrrolidinocarbonyl-3-N-pyrrolopyrazine 8 or by the intramolecular nucleophilic substitution of 2-chloro-3-(2-pyrrolyl)pyrazine 12.