383897-60-7Relevant articles and documents
Synthesis and enzymatic incorporation of a novel, bicyclic pyrimidine nucleoside: A thymidine mimic
Loakes, David,Brown, Daniel M.,Salisbury, Stephen A.,McDougall, Mark G.,Neagu, Constantin,Nampalli, Satyam,Kumar, Shiv
, p. 3387 - 3389 (2003)
Nucleophilic ring-opening and rearrangement reaction of a furanopyrimidine nucleoside with anhydrous hydrazine provided a novel, 6,6-bicyclic pyrimidopyridazin-7-one nucleoside (dH, 4), whose structure was confirmed by X-ray crystallography. This novel nucleoside was converted to its 5′-triphosphate (dHTP) for studies with DNA polymerases and incorporated into a template by using standard phosphoramidite chemistry. In the template, dH directed the incorporation of dATP and to a lesser extent dGTP into the transcript and dHTP was efficiently incorporated at the 3′-end of a primer opposite dA using both exonuclease free Klenow fragment (KF exo-) and Taq DNA polymerases and extended with natural dNTPs.
Synthesis and some biochemical properties of a novel 5,6,7,8-tetrahydropyrimido[4,5-c]pyridazine nucleoside
Loakes, David,Brown, Daniel M.,Salisbury, Stephen A.,McDougall, Mark G.,Neagu, Constantin,Nampalli, Satyam,Kumar, Shiv
, p. 1193 - 1204 (2007/10/03)
A novel nucleoside analogue is described based on the pyridazine ring system. The nucleoside was successfully incorporated into DNA via both its phosphoramidite and 5′-triphosphate derivatives. Enzymatically, the analogue behaves essentially as thymidine: