51093-31-3Relevant articles and documents
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Hems
, p. 1721 (1958)
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Simultaneous determination of five oxidative DNA lesions in human urine
Ravanat, Jean-Luc,Guicherd, Pascale,Tuce, Zorana,Cadet, Jean
, p. 802 - 808 (2007/10/03)
A method, involving a HPLC prepurification followed by a GC/MS analysis, has been set up for the measurement of nucleic acid oxidation products in human urine. For this purpose, isotopically labeled internal standards have been prepared and used for isotope dilution mass spectrometric detection. Using this approach, four oxidized DNA bases, i.e., 5-hydroxyuracil, 5- (hydroxymethyl)uracil, 8-oxo-7,8-dihydroadenine, and 8-oxo-7,8- dihydroguanine, together with 8-oxo-7,8-dihydro-2'-deoxyguanosine have been simultaneously quantified in human urine samples. The levels of the oxidized nucleic acid constituents, as expressed in picomoles per milliliter, were determined to be, in decreasing order: 8-oxo-7,8-dihydroguanine (583 ± 376) > 5-(hydroxymethyl)uracil (121 ± 56) > 5-hydroxyuracil (58 ± 23) > 8-oxo- 7,8-dihydro-2'-deoxyguanosine (30 ± 15) > 8-oxo-7,8-dihydroadenine (7 ± 4). Attempts to determine the amount of 5,6-dihydroxy-5,6-dihydrothymine, 5- hydroxycytosine, and 2,6-diamino-4-hydroxy-5-formamidopyrimidine using the above HPLC-GC/MS method were unsuccessful.
Isolation and Characterization of the Radiation-Induced Degradation Products of 2'-Deoxyguanosine in Oxygen-Free Aqueous Solutions
Berger, Maurice,Cadet, Jean
, p. 1519 - 1531 (2007/10/02)
The bulk of the radiation-induced degradation products of 2'-deoxyguanosine on oxygen-free aqueous solution have been separated by high performance liquid chromatography and characterized by various spectroscopic techniques including fast atom bombardment mass spectrometry, 1H NMR and circular dichroism.The two main decomposition products result from the opening of the imidazole ring and further rearrangement of the sugar moiety.In addition, the formation of six other nucleosides was shown to involve sugar radicals with subsequent epimerization, rearrangement or cyclization reactions. - Keywords: 2'-Deoxyguanosine, Gamma Irradiation, Hydroxyl Radicals, 1H NMR Spectra, Modified Nucleosides