518-47-8 Usage
Chemical Properties
Orange-red powder; odorless. Hygroscopic,
soluble in water; sparingly soluble in
alcohol.
Uses
Different sources of media describe the Uses of 518-47-8 differently. You can refer to the following data:
1. A sodium salt of fluorescein
2. In examining subterranean waters. Serves to ascertain source of springs, connections between streams and sea, determining approx vol of water delivered by a spring, detecting source of contamination of drinking water, infiltration of soil with waste waters of factories. In externally applied drugs and cosmetics. Analytical reagent (protein label). Clinical reagent (immuno-histological stain, immuno-fluorescent label).
3. Fluorescein sodium salt is a sodium salt of fluorescein. It can also be used in technical or engineered material use in-situ photochemical fabrication of transition metal-?promoted amorphous molybdenum sulfide catalysts for enhanced photosensitized hydrogen evolution. Dyes and metabolites, Environmental Testing
General Description
Orange-red to dark red powder. Water-soluble form of fluorescein. Odorless and almost tasteless. pH (5% solution) 7.8. May be sensitive to prolonged exposure to light and to heat.
Air & Water Reactions
Hygroscopic. Water soluble.
Reactivity Profile
Fluorescein disodium salt is incompatible with strong oxidizers. Also incompatible with acids, acid salts and salts of heavy metals .
Fire Hazard
Flash point data for Fluorescein disodium salt are not available; however, Fluorescein disodium salt is probably combustible.
Safety Profile
Moderately toxic by
intraperitoneal route. Mildly toxic by
ingestion. Human systemic effects by
intravenous route: arrhythmias, eye
hemorrhage, nausea or vomiting.
Experimental reproductive effects.
Questionable carcinogen with experimental
tumorigenic data. Mutation data reported.
When heated to decomposition it emits
toxic fumes of Na2O.
Veterinary Drugs and Treatments
Fluorescein sodium is a yellow water-soluble dye that fluoresces
under a Wood’s Lamp, but is plainly visible after binding to corneal
stroma through an ophthalmic examination light source. It is
used most commonly to delineate full thickness loss of corneal epithelium
indicating the presence of a corneal ulceration. In this instance
it will stain the corneal stroma. The epithelium is not stained
because its outer lipid cell membrane repels the stain. Descemet’s
membrane will not stain with fluorescein stain and this is used to
indicate descemetocele formation, an ocular emergency.
Fluorescein stain is applied to the precorneal tear film in dogs
and cats and the break-up of this stain with time, as observed
through a slit lamp biomicroscope using a cobalt blue light source,
is used to determine the tear film break-up time (normal 19 seconds),
an indicator of tear film quality.
Fluorescein stain is applied to the tear film of dogs to determine
patency of the nasolacrimal outflow system. The normal wait time
is 2 – 5 minutes in dogs and up to 10 minutes in cats. A positive test
indicates patency of the system. A negative test is not indicative of
disease as the test is negative in a large percentage of normal animals.
Fluorescein stain, then, can be added to irrigating solution to
flush the nasolacrimal system, making detecting the irrigation solution
at the nose more obvious during flushing of the system.
Properties and Applications
brilliant yellow. Soluble in water and ethanol (with strong green fluorescent), water-soluble good. In strong sulfuric acid with weak for fluorescence yellow, it will be diluted with yellow precipitation. Its water solution and sodium hydroxide with dark green fluorescence for brunet solution. Mainly used for sea of target that fluorescent, facilitate aerial reconnaissance search. And can use significantly for the exploration of fluorescent groundwater. Can also be used for medicine and cosmetics shading, rarely used for silk printing and dyeing things.
Check Digit Verification of cas no
The CAS Registry Mumber 518-47-8 includes 6 digits separated into 3 groups by hyphens. The first part of the number,starting from the left, has 3 digits, 5,1 and 8 respectively; the second part has 2 digits, 4 and 7 respectively.
Calculate Digit Verification of CAS Registry Number 518-47:
(5*5)+(4*1)+(3*8)+(2*4)+(1*7)=68
68 % 10 = 8
So 518-47-8 is a valid CAS Registry Number.
InChI:InChI=1/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1
518-47-8Relevant articles and documents
A highly specific fluorescent probe for hypochlorous acid and its application in imaging microbe-induced hocl production
Xu, Qingling,Lee, Kyung-Ah,Lee, Songyi,Lee, Kyung Mi,Lee, Won-Jae,Yoon, Juyoung
, p. 9944 - 9949 (2013)
Oxidative stress induced by reactive oxygen species (ROS) plays crucial roles in a wide range of physiological processes and is also implicated in various diseases, including cancer, chronic inflammatory diseases, and neurodegenerative disorders. Among the various ROS, hypochlorous acid (HOCl) plays as a powerful microbicidal agent in the innate immune system. The regulated production of microbicidal HOCl is required for the host to control the invading microbes. However, as a result of the highly reactive and diffusible nature of HOCl, its uncontrolled production may lead to an adverse effect on host physiology. Because of its biological importance, many efforts have been focused on developing selective fluorescent probes to image ROS. However, it is still challenging to design a fluorescent probe with exclusive selectivity toward a particular member of ROS. In the current work, we designed FBS as a new fluorescent HOCl probe which has high selectivity, sensitivity, and short response time in a broad range of pH. Compared with other sensors, the dual-lock structure of FBS has an advantage of eliminating interferences from other ROS/RNS. Importantly, we further showed that our HOCl probe could be applied for the in vivo imaging of physiological HOCl production in the mucosa of live animals. This probe provides a promising tool for the study of HOCl production.
